PsbS contributes to photoprotection in Chlamydomonas reinhardtii independently of energy dissipation.
PsbS contributes to photoprotection in Chlamydomonas reinhardtii independently of energy dissipation.
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PsbS 有助于莱茵衣藻的光保护，与能量耗散无关
DOI：
10.1016/j.bbabio.2020.148183
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Jahns P
中科院分区：
文献类型：
--
作者：
Redekop P;Rothhausen N;Rothhausen N;Melzer M;Mosebach L;Dulger E;Bovdilova A;Caffarri S;Hippler M;Jahns P
光合生物经常暴露在过度光照条件下，因此受到光氧化应激。为了对抗光氧化损伤，陆地植物和大多数藻类利用多余光能的非光化学猝灭（NPQ），特别是快速诱导和放松的qe机制。在维管植物中，组成活性的PsbS蛋白是qE的关键调节因子。在绿藻里。然而，reinhardtii， qE激活只有在初始高光驯化（HL）几个小时后才有可能，并且需要合成作为qE调节因子的LHCSR蛋白。PsbS在HL驯化过程中短暂表达，其具体功能尚不清楚。在此，我们研究了不同PsbS量对c .reinhardti细胞HL驯化特性的影响。研究表明，较低的PsbS含量会在不同水平上对HL驯化产生负面影响，包括NPQ容量、电子传递特性、天线组织和形态变化，导致HL敏感性总体增加，细胞活力降低。相反，更高的PsbS量不会导致更高的NPQ容量，但仍然提供更高的适应度和对HL胁迫的耐受性。引人注目的是，组成型表达的PsbS蛋白在HL驯化过程中被降解。我们认为，在HL驯化过程中，随着NPQ的激活和电子转移特性的调整，类囊体膜和/或天线蛋白的重组暂时需要PsbS，并且PsbS的降解在HL完全驯化状态下是必不可少的。
Photosynthetic organisms are frequently exposed to excess light conditions and hence to photo-oxidative stress. To counteract photo-oxidative damage, land plants and most algae make use of non- photochemical quenching (NPQ) of excess light energy, in particular the rapidly inducible and relaxing qE-mechanism. In vascular plants, the constitutively active PsbS protein is the key regulator of qE. In the green algaeC.reinhardtii, however, qE activation is only possible after initial high-light (HL) acclimation for several hours and requires the synthesis of LHCSR proteins which act as qE regulators. The precise function of PsbS, which is transiently expressed during HL acclimation inC.reinhardtii, is still unclear. Here, we investigated the impact of different PsbS amounts on HL acclimation characteristics ofC.reinhardtiicells. We demonstrate that lower PsbS amounts negatively affect HL acclimation at different levels, including NPQ capacity, electron transport characteristics, antenna organization and morphological changes, resulting in an overall increased HL sensitivity and lower vitality of cells. Contrarily, higher PsbS amounts do not result in a higher NPQ capacity, but nevertheless provide higher fitness and tolerance towards HL stress. Strikingly, constitutively expressed PsbS protein was found to be degraded during HL acclimation. We propose that PsbS is transiently required during HL acclimation for the reorganization of thylakoid membranes and/or antenna proteins along with the activation of NPQ and adjustment of electron transfer characteristics, and that degradation of PsbS is essential in the fully HL acclimated state.
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莱茵衣藻在生理相关条件下表现出事实上的 NPQ 能力1[打开]
影响因子：
7.4
作者：
W. Nawrocki;Xin Liu;R. Croce
通讯作者：
R. Croce
影响因子：
11.6
作者：
Allorent, Guillaume;Tokutsu, Ryutaro;Finazzi, Giovanni
通讯作者：
Finazzi, Giovanni
影响因子：
5.3
作者：
Schmitz J;Schöttler MA;Krueger S;Geimer S;Schneider A;Kleine T;Leister D;Bell K;Flügge UI;Häusler RE
通讯作者：
Häusler RE
DOI：
10.1111/tpj.14368
发表时间：
2019-05
期刊：
The Plant Journal
影响因子：
--
作者：
M. Scholz;Philipp Gäbelein;Huidan Xue;Laura Mosebach;S. V. Bergner;M. Hippler
通讯作者：
M. Scholz;Philipp Gäbelein;Huidan Xue;Laura Mosebach;S. V. Bergner;M. Hippler
影响因子：
4.8
作者：
Correa-Galvis, Viviana;Redekop, Petra;Jahns, Peter
通讯作者：
Jahns, Peter