β-catenin/TCF/LEF regulate expression of the short form human Cripto-1
β-catenin/TCF/LEF regulate expression of the short form human Cripto-1
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DOI：
10.1016/j.bbrc.2007.01.143
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发表时间：
2007-03-30
影响因子：
3.1
通讯作者：
Salomon, David S.
中科院分区：
文献类型：
--
作者：
Hamada, Shin;Watanabe, Kazuhide;Salomon, David S.
人畸胎瘤源性生长因子1（Teratocarcinoma derived growth factor 1，TDGF 1）/Cripto-1/CR-1基因是EGF-cripto FRL 1隐蔽基因家族的成员，在多种人类肿瘤中表达。已知大多数人结直肠肿瘤和肝癌具有组成型活性的经典Wnt/β-连环蛋白/TCF信号传导途径，也表达CR-1。结肠癌和肝癌细胞系中CR-1 mRNA短形式的表达表明，结肠癌和肝癌细胞系中经典Wnt信号通路可能对CR-1表达有差异调节。本研究表明，通过内含子-外显子增强子元件，包含三个串联TCF/LEF结合位点的CR-1基因内的经典Wnt信号通路的短形式CR-1表达的直接转录调控。(c)2007爱思唯尔公司All rights reserved.
The human gene Teratocarcinorna-derived growth factor 1 (TDGF1)/Cripto-1/CR-1 which is expressed in a wide variety of human carcinomas is a member of the EGF-cripto FRL1 cryptic (EGF-CFC) gene family. A majority of human colorectal tumors and hepatomas are known to possess a constitutively active canonical Wnt/beta-catenin/TCF signaling pathway, also express CR-1. Expression of a short form of CR-1 mRNA in colon carcinoma and hepatoma cell lines suggests that there may be differential regulation of CR-1 expression by the canonical Wnt signaling pathway in colon cancer as well as hepatoma cell lines. The present study demonstrates a direct transcriptional regulation of the short form CR-1 expression by the canonical Wnt signaling pathway through an intronic-exonic enhancer element, containing three tandem TCF/LEF binding sites within the CR-1 gene. (c) 2007 Elsevier Inc. All rights reserved.