An analysis of migratory behavior of avian cephalic neural crest cells.
An analysis of migratory behavior of avian cephalic neural crest cells.
复制标题
禽头神经嵴细胞迁移行为的分析。
DOI：
10.1016/0012-1606(75)90318-8
复制
发表时间：
1975
影响因子：
2.7
通讯作者：
D. Noden
中科院分区：
文献类型：
--
作者：
D. Noden
神经嵴细胞从它们的起源迁移到脊椎动物胚胎的许多区域。在到达终点后，这个群体的成员经历细胞分化成各种各样的细胞类型。为了追踪鸟类头神经嵴细胞的早期迁移行为，将神经褶组织从3h -胸腺嘧啶标记的供体原位移植到未标记的宿主中。随后处死宿主，用放射自显影法确定所有标记的神经嵴来源细胞的位置。嵴细胞从大脑的三个不同的preotic区域中的每一个迁移显示出独特的迁移模式，这是详细描述。在确定了这些神经嵴细胞迁移的正常模式后，提出了以下问题：发育中的神经嵴细胞群的独特迁移模式是在它们离开之前在单个细胞内决定的，还是由细胞移动的环境强加给嵴细胞的？为了解决这一问题，从上述三个颅区之一移除神经褶皱，并用来自不同头区或肱脊髓水平的3h -胸腺嘧啶标记的神经褶皱代替。在几乎所有情况下，异位移植的细胞模仿正常的迁移模式，胚胎与接受原位移植的胚胎无法区分。这证明了鸡神经嵴细胞的精确迁移模式在细胞迁移之前并不是在细胞内不可逆地决定的。相反，它们复杂而又高度组织化的迁徙行为在很大程度上受环境影响的支配。
The neural crest cells migrate from their origin into many regions of the vertebrate embryo. Upon reaching their terminal destinations, members of this population undergo cytodifferentiation into a wide range of diverse cell types. To follow the early migratory behavior of avian cephalic neural crest cells, neural fold tissue was transplanted orthotopically from a3H-thymidine-labeled donor into an unlabeled host. The hosts were sacrificed at subsequent stages and the positions of all labeled neural crest-derived cells ascertained radioautographically. Crest cells emigrating from each of three different preotic regions of the brain displayed unique patterns of migration, which are described in detail.Having established these normal patterns of neural crest cell migration, the following question was posed: Are the unique migratory patterns of the developing neural crest cell population determined within the individual cells prior to their exodus, or are they imposed upon the crest cells by the environment through which the cells move?To resolve this problem neural folds were removed from one of the three cranial regions described above and replaced with a segment of3H-thymidine-labeled neural fold from either a different cephalic region or the brachial spinal cord level. In nearly every case the heterotopically transplanted cells mimicked the normal patterns of migration, and the embryos were indistinguishable from those which had received orthotopic transplants.This proves that the precise patterns of migration of chick neural crest cells are not irreversibly determined within the cells prior to their emigration. Rather, their complex yet highly organized migratory behavior is largely directed by environmental influences.