Activation of cellular promoters during herpes virus infection of biochemically transformed cells.
Activation of cellular promoters during herpes virus infection of biochemically transformed cells.
复制标题
生化转化细胞的疱疹病毒感染期间细胞启动子的激活。
作者：
R. Everett
单纯疱疹病毒1型（HSV-1）的至少两种立即早期（IE）产物负责激活病毒早期启动子的转录。这个过程似乎不是启动子特异性的，因为几个不相关的病毒和细胞质粒携带的启动子也可以在短期转染试验中被激活。本文描述的实验表明，整合到宿主基因组中的细胞启动子也可以在病毒感染过程中被激活，这一过程是由IE基因产物引起的。在转染含有与疱疹胸苷激酶编码区（作为选择标记）连接的人β-珠蛋白启动子和兔β-珠蛋白基因的质粒后，分离生物化学转化的细胞系。用HSV-1感染一些但不是全部这样的细胞系导致对整合的β-珠蛋白和β-珠蛋白启动子的快速和显著的刺激。在伪狂犬病病毒感染期间，以及通过转染含有HSV IE基因的质粒引入后，两种启动子也可以被激活（尽管效率较低）。这些结果对病毒-宿主相互作用和病毒诱导的启动子激活机制的影响进行了讨论。
At least two of the immediate‐early (IE) products of herpes simplex virus‐1 (HSV‐1) are responsible for the activation of transcription from viral early promoters. This process appears not to be promoter specific since several unrelated viral and cellular plasmid‐borne promoters can also be activated in short‐term transfection assays. This paper describes experiments that show that cellular promoters integrated into the host genome can also be activated during viral infection, and that this process is brought about by IE gene products. Biochemically transformed cell lines were isolated following transfection of plasmids containing the human epsilon‐globin promoter linked to the herpes thymidine kinase coding region (as selectable marker), and an unselected rabbit beta‐globin gene. Infection of some, but not all, such cell lines with HSV‐1 resulted in a rapid and considerable stimulation of the integrated epsilon‐ and beta‐globin promoters. Both promoters could also be activated (albeit less efficiently) during pseudorabies virus infection, and after introduction by transfection of plasmids containing HSV IE genes. The implications of these results for viral‐host interactions and the mechanism of viral‐induced promoter activation are discussed.