Both early and late apoptotic blebs are taken up by DC and induce IL-6 production
Both early and late apoptotic blebs are taken up by DC and induce IL-6 production
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DOI：
10.1080/08916930902828049
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发表时间：
2009-01-01
期刊：
影响因子：
3.5
通讯作者：
Van Der Vlag, J.
中科院分区：
文献类型：
--
作者：
Fransen, J. H.;Hilbrands, L. B.;Van Der Vlag, J.
在脱泡过程中，在细胞表面形成含有核成分的泡。当这些水泡从垂死的细胞中分离出来时，载脂蛋白细胞体仍然存在。载脂蛋白滤过泡的内容物被修饰并且可以被释放，特别是在系统性红斑狼疮（SLE）患者中，因为在这种自身免疫性疾病中观察到载脂蛋白物质的清除受损。因此，存在于SLE受试者中的自身抗体与载脂蛋白滤过泡结合。基于AnxA 5结合和PI的渗透性，我们表明脱辅基泡可以分为早期（AnxA 5（+）/PI-）或晚期（AnxA 5（+）/PI+）脱辅基泡。这两种形式的水泡含有载脂蛋白诱导的染色质修饰，并有效地吞噬树突状细胞（DC）。晚期和早期脱泡的DC摄取刺激DC产生IL-6。这种水泡诱导的DC效应可能是SLE自身免疫反应启动的重要步骤。
During apo blebs, containing nuclear components, are formed at the cells' surfaces. When these blebs separate from the dying cell an apo cell body remains. The contents of apo blebs are modified and can be released, especially in patients with systemic lupus erythematosus (SLE) since impaired clearance of apo material has been observed in this autoimmune condition. Accordingly, autoantibodies present in subjects with SLE bind to apo blebs. Based on AnxA5 binding, and permeability for PI, we show that apo blebs can be categorized as early (AnxA5(+)/PI-) or late (AnxA5(+)/PI+) apo ones. Both forms of blebs contain apo-induced chromatin modifications and are efficiently phagocytosed by dendritic cell (DC). Uptake by DC of late, but also early apo blebs, stimulate DC to produce IL-6. This bleb-induced effect on DC may be an important step in the initiation of the autoimmune responses in SLE.