Differential Development of Glucose Intolerance and Pancreatic Islet Adaptation in Multiple Diet Induced Obesity Models
Differential Development of Glucose Intolerance and Pancreatic Islet Adaptation in Multiple Diet Induced Obesity Models
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DOI：
10.3390/nu4101367
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发表时间：
2012-10-01
期刊：
影响因子：
5.9
通讯作者：
Ahren, Bo
中科院分区：
文献类型：
--
作者：
Omar, Bilal;Pacini, Giovanni;Ahren, Bo
背景：高脂饮食的C57BL/6小鼠是肥胖和2型糖尿病（T2D）实验研究中常见且有价值的模型。研究人员使用了不同的高脂肪饮食，为了确定哪种饮食产生的模型最接近人类的T2D，需要对它们进行全面比较。方法：采用糖耐量试验（IVGTT）和正糖夹钳法，比较C57BL/6J小鼠60%高脂饮食（HFD）和58%高脂高糖苏威特饮食（HFHS）及其对照的4种不同饮食。结果：在能量摄入相似的情况下，喂食高脂食物的小鼠比喂食高脂食物的小鼠体重增加更多。8周后，两种高脂饮食模型均出现葡萄糖不耐受。喂食HFD的小鼠有升高的基础胰岛素，这在HFHS组中没有出现。HFD组的急性胰岛素反应（AIR）没有变化，但HFHS饮食组的急性胰岛素反应（AIR）略有增加。在IVGTT期间，HFHS饮食组的胰岛素总分泌量是对照组的三倍，而HFD组没有发现差异。高脂饮食组的胰岛素敏感性降低了四倍，而高脂饮食组没有。结论：HFD和HFHS饮食模型对胰岛素抵抗和β细胞适应的发展有不同的影响。为了在特定研究中选择合适的饮食，认识到这些差异是很重要的。
Background: The C57BL/6 mouse fed a high fat diet is a common and valuable model in experimental studies of obesity and type 2 diabetes (T2D). Different high fat diets are used and in order to determine which diet produces a model most accurately resembling human T2D, they need to be compared head-to-head. Methods: Four different diets, the 60% high fat diet (HFD) and the 58% high fat-high sucrose Surwit diet (HFHS) and their respective controls, were compared in C57BL/6J mice using glucose tolerance tests (IVGTT) and the euglycemic clamp. Results: Mice fed a HFD gained more weight than HFHS fed mice despite having similar energy intake. Both high fat diet models were glucose intolerant after eight weeks. Mice fed the HFD had elevated basal insulin, which was not seen in the HFHS group. The acute insulin response (AIR) was unchanged in the HFD group, but slightly increased in the HFHS diet group. The HFHS diet group had a threefold greater total insulin secretion during the IVGTT compared to its control, while no differences were seen in the HFD group. Insulin sensitivity was decreased fourfold in the HFD group, but not in the HFHS diet group. Conclusion: The HFD and HFHS diet models show differential effects on the development of insulin resistance and beta cell adaptation. These discrepancies are important to acknowledge in order to select the appropriate diet for specific studies.