Novel repeats in the genome of the woodchuck Marmota monax.
Novel repeats in the genome of the woodchuck Marmota monax.
复制标题
土拨鼠 Marmota monax 的基因组中出现了新的重复。
DOI：
10.1093/nar/19.11.3151
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发表时间：
1991
影响因子：
14.9
通讯作者：
Miller,RH
中科院分区：
文献类型：
--
作者：
Jing,X;Miller,RH
Infection of woodchucks from the Eastern United States (Marmota monax) with the woodchuck hepatitis virus (WHV) has proven to be an excellent animal model system for the study of hepatitis B virus infection of humans. Woodchucks infected experimentally with WHV develop acute virus infections which can become chronic infections leading to hepatocellular carcinoma (1-7). However, understanding hepatocarcinogenesis using this model system is limited by a general lack of information on the host genome.In this study we cloned DNA from the genome ofan uninfected woodchuck to search for unique, repeated DNA sequences. Genomic DNA was isolated from woodchuck number 809, digested with restriction endonuclease HindI, and cloned into the HindI site ofpUC13 DNA. Independent recombinants were selected for analysis. Recombinant W220172, a 318 bp HindIl fragment, was found to occur 20 times in the woodchuck genome using standard hybridization techniques (8). Recombinant W87, a 249 bp repeat, was found to be repeated approximately 1,000 times in the woodchuck genome. Both repeats lack significant open reading frames and are AT rich (Table 1). Computer analysis (9) indicates that there are no sequences in the EMBL 25 or the GenBank 66 databases that possess statistically significant sequence similarity with the two repeats. Thus, these repeats appear to be unique. Repeat W87 may be useful as a probe to identify woodchuck specific DNA sequences in mouse NIH 3T3 cell assays in the search for oncogenes responsible for liver tumor formation in WHV-infected animals.
