DISPENSABLE SEQUENCES AND PACKAGING CONSTRAINTS OF DNA FROM THE STREPTOMYCES TEMPERATE PHAGE PHI-C31
DISPENSABLE SEQUENCES AND PACKAGING CONSTRAINTS OF DNA FROM THE STREPTOMYCES TEMPERATE PHAGE PHI-C31
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DOI：
10.1016/0378-1119(81)90134-7
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发表时间：
1981-01-01
期刊：
影响因子：
3.5
通讯作者：
SUAREZ, JE
中科院分区：
文献类型：
--
作者：
CHATER, KF;BRUTON, CJ;SUAREZ, JE
通过2种方法选择温和链霉菌噬菌体vphi C31的缺失突变体：对螯合剂焦磷酸钠的抗性和在S. albus G获得大噬斑突变体。除了包括阻遏物基因的较短片段之外，缺失限定了vphi C31基因组的7.7 kb的片段，其对于噬斑形成是不必要的。缺失和插入的分析表明，允许噬斑形成的v φ C31基因组的最小尺寸为37.5kb（野生型长度41.2kb的91%），最大尺寸为至少42.4kb（103%）。应该可以将多达10kb的外源DNA导入适当开发的v φ C31载体中。
Deletion mutants of the temperate Streptomyces phage .vphi.C31 were selected by 2 methods: resistance to the chelating agent sodium pyrophosphate and plating of a .vphi.C31::pBR322 hybrid phage on S. albus G to obtain large plaque mutants. The deletions defined a 7.7 kilobase (kb) segment of the .vphi.C31 genome which is inessential for plaque formation, in addition to a shorter segment including the repressor gene. Analysis of deletions and insertions suggested that the minimum size of the .vphi.C31 genome allowing plaque formation is 37.5 kb (91% of the wild-type length of 41.2 kb), and the maximum is at least 42.4 kb (103%). It should be possible to introduce up to 10 kb of foreign DNA into a suitably developed .vphi.C31 vector.