Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations
Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations
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成年猕猴海马的单细胞转录组学揭示了神经前体细胞群
DOI：
10.1038/s41593-022-01073-x
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发表时间：
2022-05-30
影响因子：
25
通讯作者：
Liu, Sheng
中科院分区：
文献类型：
--
作者：
Hao, Zhao-Zhe;Wei, Jia-Ru;Liu, Sheng
神经发生在成年灵长类动物中的程度仍然存在争议。在这项研究中，使用优化的单细胞RNA测序管道，我们分析了来自成年猕猴海马的207，785个细胞，并鉴定了34个细胞群，包括所有主要的海马细胞类型。对它们的基因表达、特化轨迹和基因调控网络的分析揭示了所有关键神经源性前体细胞群体的存在，包括放射状胶质样细胞（RGL）、中间祖细胞（IPC）和成神经细胞的异质池。HMGB2是一种新的IPC标志物。与小鼠单细胞转录组学数据的比较揭示了物种间神经发生过程的差异。我们证实，在成年灵长类动物的离体神经球培养物中，神经发生被重演，进一步支持了能够增殖和分化的神经前体细胞（NPC）的存在。我们的大规模数据集为灵长类动物提供了一个全面的成年神经发生图谱。
The extent to which neurogenesis occurs in adult primates remains controversial. In this study, using an optimized single-cell RNA sequencing pipeline, we profiled 207,785 cells from the adult macaque hippocampus and identified 34 cell populations comprising all major hippocampal cell types. Analysis of their gene expression, specification trajectories and gene regulatory networks revealed the presence of all key neurogenic precursor cell populations, including a heterogeneous pool of radial glia-like cells (RGLs), intermediate progenitor cells (IPCs) and neuroblasts. We identifiedHMGB2as a novel IPC marker. Comparison with mouse single-cell transcriptomic data revealed differences in neurogenic processes between species. We confirmed that neurogenesis is recapitulated in ex vivo neurosphere cultures from adult primates, further supporting the existence of neural precursor cells (NPCs) that are able to proliferate and differentiate. Our large-scale dataset provides a comprehensive adult neurogenesis atlas for primates.