Isolation of Porphyromonas gingivalis and detection of immunoglobulin a specific to fimbrial antigen in gingival crevicular fluid
Isolation of Porphyromonas gingivalis and detection of immunoglobulin a specific to fimbrial antigen in gingival crevicular fluid
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DOI：
10.1128/jcm.36.8.2322-2325.1998
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发表时间：
1998-08-01
影响因子：
9.4
通讯作者：
Lo Bue, AM
中科院分区：
文献类型：
--
作者：
Condorelli, F;Scalia, G;Lo Bue, AM
本研究评价了牙龈卟啉单胞菌的患病率以及细菌培养方法与龈沟液（GCF）中牙龈卟啉单胞菌菌毛抗原特异性免疫球蛋白A（伊加）检测之间的相关性。牙龈卟啉单胞菌分离自牙周病患者活动部位龈下菌斑样本的78.3%和非活动部位龈下菌斑样本的34.7%。牙龈卟啉单胞菌分离自仅4.7%的健康受试者（对照组）。采用酶联免疫吸附试验（ELISA）检测牙龈卟啉单胞菌菌毛抗原特异性免疫球蛋白。牙龈卟啉单胞菌培养方法的结果和牙周病患者的特异性伊加检测结果之间的总体一致性为71.7%的活动部位和58.7%的非活动部位。牙龈卟啉单胞菌特异性伊加在健康受试者的所有部位的GCF中均不存在。结果表明，牙龈卟啉单胞菌与局部产生特异性伊加球蛋白A有关，通过ELISA检测GCF中牙龈卟啉单胞菌特异性伊加抗体可作为揭示复发性牙周感染激活早期阶段的预测参数。
The present study evaluated the prevalence of Porphyromonas gingivalis and the correlation between the bacterial culture method and the detection of immunoglobulin A (IgA) specific to the P. gingivalis fimbrial antigen in gingival crevicular fluid (GCF). P.gingivalis was isolated from 78.3% of subgingival plaque samples obtained from active sites and 34.7% of those from inactive sites of periodontal patients. P. gingivalis was isolated from only 4.7% of healthy subjects (control group). Immunoglobulins specific to the P. gingivalis fimbrial antigen were detected by enzyme-linked immunosorbent assay (ELISA). The overall agreement between the results of the P. gingivalis culture method and the results of specific IgA detection in periodontal patients was 71.7% for active sites and 58.7% for inactive sites. IgA specific to P. gingivalis was absent in GCF from all of the sites of healthy subjects. The results suggest that P. gingivalis is associated with the local production of specific IgA, The detection of IgA antibodies specific to P. gingivalis in GCF by ELISA may be used as a predictive parameter to reveal the early phase of the activation of recurrent periodontal infections.