Tumor-specific antigen of murine T-lymphoma defined with monoclonal antibody.
Tumor-specific antigen of murine T-lymphoma defined with monoclonal antibody.
复制标题
DOI：
10.4049/jimmunol.129.5.2293
复制
发表时间：
1982-11
影响因子：
4.4
通讯作者：
James P. Allison;Bradley W. McIntyre;D. Bloch
中科院分区：
文献类型：
--
作者：
James P. Allison;Bradley W. McIntyre;D. Bloch
将P3X63Ag8骨髓瘤细胞与免疫C57BL/Ka x射线诱导淋巴瘤（C6XL）的BALB/c小鼠的脾脏细胞融合，构建杂交瘤细胞板。43个杂交瘤中有一个分泌抗体与肿瘤细胞反应，在放射免疫测定中发现与正常脾细胞无反应。该抗体编号124-40，在放射免疫测定或放射免疫沉淀试验中对正常成人胸腺、脾脏、淋巴结或骨髓细胞，或胎儿脾脏或胸腺细胞无反应。这些非恶性淋巴样细胞的流动微荧光分析未能显示与MAb 124-40反应的亚群。该抗体对用于免疫的淋巴瘤细胞具有高度特异性，并且与其他自发或x射线诱导或化学诱导的淋巴瘤不发生反应。用放射免疫沉淀法分离出与MAb 124-40有反应的抗原，发现是一个由39000和41000 m.w的二硫键结合亚基组成的糖蛋白。纯化后的T细胞提取物可通过双向电泳检测到结构相似但与MAb 124-40无反应的细胞表面成分，但B细胞未检测到。这些结果表明，与MAb 124-40反应的明显个体特异性淋巴瘤抗原可能是由T细胞表面组分携带的克隆表达表位。
A panel of hybridomas was constructed by fusion of P3X63Ag8 myeloma cells with spleen cells from a BALB/c mouse that had been immunized with a C57BL/Ka x-ray-induced lymphoma, C6XL. One of forty-three hybridomas secreting antibodies reactive with the tumor cells was found to be unreactive with normal spleen cells in a radioimmunometric assay. This antibody, designated 124-40, was unreactive with normal adult thymus, spleen, lymph node, or bone marrow cells, or with fetal spleen or thymus cells in radioimmunometric or radioimmunoprecipitation assays. Flow microfluorometric analysis of these nonmalignant lymphoid cells failed to reveal subpopulations reactive with MAb 124-40. The antibody was highly specific for the lymphoma cells used for immunization and did not react with a panel of other spontaneous or x-ray-induced or chemically induced lymphomas. The antigen reactive with MAb 124-40 was isolated by radioimmunoprecipitation and found to be a glycoprotein composed of disulfide-bonded subunits of 39,000 m.w. and 41,000 m.w. A cell surface component of similar structure, but not reactive with MAb 124-40 could be detected by two-dimensional electrophoresis in extracts of purified T cells, but not B cells. These results suggest that the apparently individually specific lymphoma antigen reactive with MAb 124-40 might be a clonally expressed epitope carried by a T cell surface component.