Spontaneous change of a benign satellite RNA of cucumber mosaic virus to a pathogenic variant
Spontaneous change of a benign satellite RNA of cucumber mosaic virus to a pathogenic variant
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DOI：
10.1038/nbt1096-1264
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发表时间：
1996-10-01
影响因子：
46.9
通讯作者：
Roossinck, MJ
中科院分区：
文献类型：
--
作者：
Palukaitis, P;Roossinck, MJ
植物卫星RNA通常降低辅助病毒积累的水平，并减弱由辅助病毒诱导的疾病症状，它们依赖于辅助病毒进行复制和包装。因此，卫星RNA可以用作生物控制剂以降低大田作物中的疾病水平，通过将病毒疫苗应用于健康植物，或者通过卫星RNA在转化植物中的转基因表达。已经在田间试验中使用的一种这样的病毒/卫星RNA系统是黄瓜花叶病毒（CMV）及其卫星RNA。然而，在这个系统中，一些卫星RNA也加剧了特定宿主植物中的病毒病。我们将CMV的卫星RNA与其辅助病毒一起传代，以确定减弱烟草植物上CMV诱导的疾病的卫星RNA是否可以突变为致病形式，然后可以选择。在涉及来自两个亚组中的每一个的CMV菌株的几个实验中，卫星迅速突变为致病形式，其被选择。这证明了与使用减毒卫星RNA作为CMV生物防治形式相关的固有风险。
Plant satellite RNAs generally reduce the level of helper virus accumulation and attenuate the disease symptoms induced by the helper virus that they depend upon for replication and packaging. As such, satellite RNAs could be used as biocontrol agents to reduce the level of disease in field crops, either by the application of a viral vaccine to healthy plants, car by the transgenic expression of satellite RNA in transformed plants. one such virus/satellite RNA system already under use in field tests is cucumber mosaic virus (CMV) and its satellite RNAs. However, in this system, some satellite RNAs also intensify viral disease in particular host plants. We passaged a satellite RNA of CMV with its helper virus to determine whether a satellite RNA that attenuates CMV-induced disease on tobacco plants could mutate to a pathogenic form, which might then be selected. In several experiments involving strains of CMV from each of the two subgroups, the satellite rapidly mutated to a pathogenic form, which was selected. This demonstrates an inherent risk associated with the use of attenuating satellite RNAs as a form of biocontrol of CMV.