Neuronal and behavioural modulations by pathway-selective optogenetic stimulation of the primate oculomotor system.
Neuronal and behavioural modulations by pathway-selective optogenetic stimulation of the primate oculomotor system.
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DOI：
10.1038/ncomms9378
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发表时间：
2015-09-21
影响因子：
16.6
通讯作者：
Matsumoto M
中科院分区：
文献类型：
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作者：
Inoue KI;Takada M;Matsumoto M
光遗传学能够在时间和空间上精确控制体内神经元的活动。光遗传学的一个关键优势是它可以用来控制连接特定大脑区域的靶向神经通路的活动。虽然这种路径选择性光遗传学控制是啮齿动物中的流行工具，但使用路径选择性光遗传学调节行为的尝试在灵长类动物中尚未成功。在这里，我们开发了一种方法，在猕猴的路径选择性光遗传学，专注于从额眼场（FEF）的上级丘（SC），复杂的眼球网络的一部分的途径。我们发现，光遗传学刺激的FEF预测SC调制SC神经元的活动，并足以引起扫视眼球运动的响应领域对应的刺激部位。因此，我们的结果证明了使用路径选择性光遗传学来阐明灵长类动物神经网络功能的可行性。 通路选择性光遗传学能够精确控制连接特定大脑区域的靶向通路中的神经活动。在这里，作者首次证明了路径选择性光遗传学可以调节非人类灵长类动物的神经活动和行为。
Optogenetics enables temporally and spatially precise control of neuronal activity in vivo. One of the key advantages of optogenetics is that it can be used to control the activity of targeted neural pathways that connect specific brain regions. While such pathway-selective optogenetic control is a popular tool in rodents, attempts at modulating behaviour using pathway-selective optogenetics have not yet been successful in primates. Here we develop a methodology for pathway-selective optogenetics in macaque monkeys, focusing on the pathway from the frontal eye field (FEF) to the superior colliculus (SC), part of the complex oculomotor network. We find that the optogenetic stimulation of FEF projections to the SC modulates SC neuron activity and is sufficient to evoke saccadic eye movements towards the response field corresponding to the stimulation site. Thus, our results demonstrate the feasibility of using pathway-selective optogenetics to elucidate neural network function in primates. Pathway-selective optogenetics enables the precise control of neural activity in targeted pathways connecting specific brain regions. Here the authors provide the first demonstration that pathway-selective optogenetics can modulate neural activity and behaviour in non-human primates.