Deforestation fosters bacterial diversity and the cyanobacterial community responsible for carbon fixation processes under semiarid climate: a metaproteomics study
Deforestation fosters bacterial diversity and the cyanobacterial community responsible for carbon fixation processes under semiarid climate: a metaproteomics study
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DOI：
10.1016/j.apsoil.2015.04.006
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发表时间：
2015-09-01
影响因子：
4.8
通讯作者：
Jehmlich, Nico
中科院分区：
文献类型：
--
作者：
Bastida, Felipe;Garcia, Carlos;Jehmlich, Nico
森林砍伐对土壤微生物群落及其功能的长期影响基本上是未知的。为了同时评估的生物学和功能，我们应用磷脂脂肪酸分析（PLFA），元蛋白质组学和酶活性的土壤样品中的自然区域位于西班牙东南部（卡拉瓦卡德拉克鲁斯），占主导地位的松halepensis（F），和邻近地区砍伐森林15年前（DF）。森林砍伐导致细菌生物量和酶活性的长期损失，但增加了细菌的多样性，估计由元蛋白质组学。蛋白质丰度分析表明，变形菌在F中的丰度高于DF。此外，蓝藻蛋白的丰度是显着较高的DF（7.3%）相比，F（0.9%）。有趣的是，蓝藻蛋白质参与碳固定（核酮糖1，5-二磷酸羧化酶，藻蓝蛋白和光系统蛋白）只在DF中被确定。这些数据表明，蓝藻在半干旱地区森林砍伐后的生态系统功能和生物固碳中起着关键作用。(C)2015 Elsevier B. V.版权所有。
The long-term effects of deforestation on the soil microbial community and its functionality are largely unknown. In order to assess simultaneously the phylogeny and functionality, we applied phospholipid fatty acid analysis (PLFAs), metaproteomics and enzyme activities in soil samples from a natural area located in Southeast Spain (Caravaca de la Cruz), dominated by Pinus halepensis (F), and an adjacent area deforested 15-years ago (DF). Deforestation induced a long-term loss of bacterial biomass and enzyme activity, but an increase in the bacterial diversity as estimated by metaproteomics. Protein abundances analysis revealed that Proteobacteria was higher in F than DF. In addition, the abundance of cyanobacterial proteins was significantly higher in DF (7.3%) when compared to F (0.9%). Interestingly, cyanobacterial proteins involved in carbon fixation (Ribulose 1,5-bisphosphate carboxylase, phycocyanins and photosystem proteins) were only identified in DF. The data suggest that Cyanobacteria play a critical role in the ecosystem functioning and biotic carbon fixation when soil is deforested in semiarid areas. (C) 2015 Elsevier B.V. All rights reserved.