Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs.
Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs.
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DOI：
10.2527/2001.792347x
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发表时间：
2001-02
影响因子：
3.3
通讯作者：
F. Gerbens;F. Verburg;H. V. van Moerkerk;B. Engel;W. Buist;J H Veerkamp;M F te Pas
中科院分区：
文献类型：
--
作者：
F. Gerbens;F. Verburg;H. V. van Moerkerk;B. Engel;W. Buist;J H Veerkamp;M F te Pas
肌内脂肪含量是决定猪肉品质的主要因素。此前，脂肪细胞和心脏脂肪酸结合蛋白基因A-FABP和H-FABP的多态与杜洛克猪群体肌内脂肪含量的遗传变异显著相关。在梅山杂交群体中，进一步支持H-FABP的作用，而不支持A-FABP的作用。然而，在这些分析中不能排除紧密连锁的基因的影响。为了验证A-FABP和H-FABP在肌内脂肪积累中的作用，对153头杂交猪的A-FABP和H-FABP基因的多态性、FABP基因的mRNA和蛋白表达水平以及腰最长肌的肌内脂肪含量进行了研究。对于H-FABP，统计分析显示H-FABP HaeIII-RFLP基因型之间在mRNA表达水平上存在显著差异，但在蛋白表达水平上没有显著差异。在这些基因型之间，公猪的肌内脂肪含量存在显著差异，而母猪的肌内脂肪含量没有显著差异。此外，H-FABP的mRNA表达水平与肌内脂肪含量显著相关，而蛋白表达水平与肌内脂肪含量无关。在A-FABP基因分型中，基因表达水平和蛋白表达水平无显著差异。然而，公猪的肌内脂肪含量存在显著差异，而母猪的差异不显著。此外，A-FABP mRNA的表达水平与肌内脂肪含量显著相关，而不是蛋白质表达水平。综上所述，肌内脂肪含量的变化不能用A-FABP和H-FABP基因和蛋白表达水平的差异来解释。然而，这可能是由于使用的化验方法的局限性和(或)采样时间的不适当。最后，结果表明A-FABP和H-FABP的表达是翻译调控的，而不是转录调控的。
Intramuscular fat content is a major determinant of meat quality in pigs. Previously, polymorphisms in the adipocyte and heart fatty acid-binding protein genes, A-FABP and H-FABP, have been significantly associated with genetic variation of intramuscular fat content in a Duroc pig population. Further support for the role of H-FABP but not for A-FABP was found in a Meishan crossbred population. However, the effect of closely linked genes could not be excluded in these analyses. To validate the role of A-FABP and H-FABP in intramuscular fat accretion, 153 pigs of a crossbred genotype were evaluated for the A-FABP and H-FABP polymorphisms, mRNA, and protein expression levels of both FABP genes and intramuscular fat content in the longissimus lumborum muscle. For H-FABP, statistical analyses showed significant differences in mRNA but not protein expression levels between H-FABP HaeIII PCR-RFLP genotype classes. Between these genotype classes, significant differences in intramuscular fat content were found within barrows but not in gilts. Moreover, H-FABP mRNA but not protein expression levels were significantly related to intramuscular fat content. For A-FABP genotype classes, no significant differences in mRNA and protein expression levels were found. However, a significant difference in intramuscular fat content was found within barrows but not in gilts. In addition, a significant relationship between A-FABP mRNA but not protein expression levels and intramuscular fat content was found. In conclusion, variation of intramuscular fat content could not be explained by differences in A-FABP and H-FABP mRNA and protein expression levels. However, this may be due to limitations of the assays used and(or) the inappropriateness of the time of sampling. Finally, results suggest that A-FABP and H-FABP expression are translationally rather than transcriptionally regulated.