Arbuscular mycorrhizal fungi effect growth and photosynthesis of Phragmites australis (Cav.) Trin ex. Steudel under copper stress
Arbuscular mycorrhizal fungi effect growth and photosynthesis of Phragmites australis (Cav.) Trin ex. Steudel under copper stress
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丛枝菌根真菌影响芦苇 (Phragmites australis) (Cav.) Trin ex. 的生长和光合作用。
作者：
Wu J T;Wang L;Zhao L;Huang X C;Ma F
丛枝菌根真菌（AMF）是解除重金属对植物生长抑制的有效途径，但目前还没有研究AMF对重金属胁迫下芦苇（Phragmites australis）耐铜性增强相关生理生化变化的影响。研究了不同浓度铜胁迫下，不同生长发育参数和光合特性的变化。利用同位素标记相对和绝对定量（iTRAQ）系统对差异表达蛋白进行定量分析，发现接种AMF可以缓解铜胁迫对光合作用的抑制。澳大利亚，从而促进增长。蛋白质组学分析结果表明，在铜胁迫下，接种红曲霉的蛋白质表达量显著增加。在根芽中，不调节蛋白导致总共459种不同表达的蛋白（200种上调和259种下调）。此外，AMF诱导的光合作用变化主要涉及跨膜蛋白-色素复合物CP 43（光系统II）和FNR（铁氧还蛋白-NADP+光合电子传递相关氧化还原酶）的表达上调，表明AMF能有效促进P.从而为阐明AMF增强植物耐铜性的机制提供了新的方向和指导性依据。
Arbuscular mycorrhizal fungi (AMF) is an effective way to remove heavy metals’ inhibition on plants, however, few relevant research attempts have been made to determine the contribution of AMF to the physiological and biochemical changes related to the enhanced copper tolerance ofPhragmites australisunder metal‐stressed conditions.In this study, the effects of AMF inoculation onP. australisunder different concentrations of copper stress were investigated according to the changes in the parameters related to growth and development, and photosynthetic charateristics. Then, differentially expressed proteins (DEPs) were evaluated by the Isobaric Tag for Relative and Absolute Quantification (iTRAQ) system, which could accurately quantify the DEPs by measuring peak intensities of reporter ions in tandem mass spectrometry (MS/MS) spectra.It was found that AMF inoculation may relieve the photosynthesis inhibition caused by copper stress onP. australisand thus promote growth. Proteomic analysis results showed that under copper stress, the inoculation ofR. irregularisresulted in a total of 459 differently‐expressed proteins (200 up‐regulated and 259 down‐regulated) in root buds. In addition, the photosynthetic changes caused by AMF inoculation mainly involve the up‐regulated expression of transmembrane protein–pigment complexes CP43 (photosystem II) and FNR (ferredoxin‐NADP+ oxidoreductase related to photosynthetic electron transport).These results indicate that AMF could effectively improve the growth and physiological activity ofP. australisunder copper stress, and thus provides a new direction and instructive evidence for determining the mechanisms by which AMF inoculation enhances the copper tolerance of plants.