Detection of the parasitic dinoflagellate Hematodinium in the Norway lobster Nephrops norvegicus by ELISA.

Detection of the parasitic dinoflagellate Hematodinium in the Norway lobster Nephrops norvegicus by ELISA.
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DOI:
10.3354/dao052175
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发表时间:
2002-11
影响因子:
1.4
通讯作者:
HJ Small;S. Wilson;DM Neil;P. Hagan;GH Coombs
HJ Small;S. Wilson;DM Neil;P. Hagan;GH Coombs
中科院分区:
农林科学3区
文献类型:
--
作者:
HJ Small;S. Wilson;DM Neil;P. Hagan;GH Coombs

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来自苏格兰沿海沃茨的挪威龙虾Nephrops norvegicus季节性感染血甲藻属的寄生甲藻。建立了检测血淋巴中寄生虫的酶联免疫吸附试验(ELISA)。挪威人。ELISA方法操作简单,检测限为5 × 10(4)个寄生虫ml(-1)血淋巴。ELISA法目前被用于研究血吸虫感染在N。norvegicus和其他甲壳类宿主。
Norway lobsters Nephrops norvegicus from the coastal waters of Scotland are seasonally infected by a parasitic dinoflagellate of the genus Hematodinium. An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of the parasite in the haemolymph of N. norvegicus. The ELISA is simple to perform with a detection limit of 5 x 10(4) parasites ml(-1) haemolymph. The ELISA is currently being used to study the prevalence and seasonality of Hematodinium infection in N. norvegicus and other crustacean hosts.