Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification
Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification
复制标题
通过将细菌变构转录因子的识别与等温链置换扩增相结合来开发小分子生物传感器。
DOI：
10.1039/c8cc01764f
复制
发表时间：
2018
影响因子：
4.9
通讯作者：
Wang Weishan
中科院分区：
文献类型：
--
作者：
Yao Yongpeng;Li Shanshan;Cao Jiaqian;Liu Weiwei;Fan Keqiang;Xiang Wensheng;Yang Keqian;Kong Deming;Wang Weishan
在这里，我们通过将细菌变构转录因子（aTF）的小分子识别与体外等温链置换扩增（SDA）相结合，展示了一种易于实施且通用的生物传感策略。基于该策略，我们分别使用细菌aTF HosA和HucR开发了两种生物传感器，用于检测防腐剂对羟基苯甲酸和疾病标志物尿酸，凸显了该策略在小分子生物传感器开发方面的巨大潜力。
Here, we demonstrate an easy-to-implement and general biosensing strategy by coupling the small-molecule recognition of the bacterial allosteric transcription factor (aTF) with isothermal strand displacement amplification (SDA) in vitro. Based on this strategy, we developed two biosensors for the detection of an antiseptic, p-hydroxybenzoic acid, and a disease marker, uric acid, using bacterial aTF HosA and HucR, respectively, highlighting the great potential of this strategy for the development of small-molecule biosensors.