Membrane-partitioned cell wall synthesis in mycobacteria.
Membrane-partitioned cell wall synthesis in mycobacteria.
复制标题
分枝杆菌的膜分隔细胞壁合成。
DOI：
10.7554/elife.60263
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发表时间：
2021-02-05
期刊：
影响因子：
7.7
通讯作者：
Siegrist MS
中科院分区：
文献类型：
--
作者：
García-Heredia A;Kado T;Sein CE;Puffal J;Osman SH;Judd J;Gray TA;Morita YS;Siegrist MS
许多抗生素的目标是细胞壁肽聚糖的组装，肽聚糖是包裹大多数细菌的基本杂聚网。在杆状细菌中，细胞壁的延伸在空间上是精确的，但依赖于有限的脂质连接的前体池，这些前体产生并被吸引到膜紊乱。通过跟踪酶，底物和肽聚糖的合成产物在耻垢分枝杆菌，我们表明，前体是在质膜结构域，是横向和生化不同的网站的细胞壁组装。膜分配可能有助于稳健、有序的肽聚糖合成，表明这些结构域有助于模板肽聚糖合成。细胞壁组织蛋白DivIVA和细胞壁本身促进结构域稳态。这些数据支持一个模型，其中肽聚糖聚合物反馈其膜模板，以保持有利于定向合成的环境。我们的研究结果适用于杆状细菌，这些细菌在遗传学上与M. smegaims，表明前体的水平区室化可能是杆菌细胞壁生物发生的一般特征。
Many antibiotics target the assembly of cell wall peptidoglycan, an essential, heteropolymeric mesh that encases most bacteria. In rod-shaped bacteria, cell wall elongation is spatially precise yet relies on limited pools of lipid-linked precursors that generate and are attracted to membrane disorder. By tracking enzymes, substrates, and products of peptidoglycan biosynthesis in Mycobacterium smegmatis, we show that precursors are made in plasma membrane domains that are laterally and biochemically distinct from sites of cell wall assembly. Membrane partitioning likely contributes to robust, orderly peptidoglycan synthesis, suggesting that these domains help template peptidoglycan synthesis. The cell wall-organizing protein DivIVA and the cell wall itself promote domain homeostasis. These data support a model in which the peptidoglycan polymer feeds back on its membrane template to maintain an environment conducive to directional synthesis. Our findings are applicable to rod-shaped bacteria that are phylogenetically distant from M. smegmatis, indicating that horizontal compartmentalization of precursors may be a general feature of bacillary cell wall biogenesis.