An efficient and robust fatty acid profiling method for plasma metabolomic studies by gas chromatography-mass spectrometry
An efficient and robust fatty acid profiling method for plasma metabolomic studies by gas chromatography-mass spectrometry
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DOI：
10.1016/j.cca.2015.09.028
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发表时间：
2015-12-07
影响因子：
5
通讯作者：
Kuo, Ching-Hua
中科院分区：
文献类型：
--
作者：
Chiu, Huai-Hsuan;Tsai, Sung-Jeng;Kuo, Ching-Hua
背景：脂肪酸的靶向代谢组学分析已将脂肪酸调节异常与许多疾病联系起来。本研究选择了5种常用的脂肪酸衍生化方法进行比较。方法：比较方法的精密度和衍生化效率，选择最经济、效果最好的方法进行方法学验证。24个脂肪酸标准品被用来验证的方法，这是后来应用于乳腺癌的潜在脂肪酸标志物的调查。结果：乙酰氯方法被证明提供最好的衍生效率和最低的成本为血浆样品。在优化条件下，离子液体色谱柱在26 min内成功分离了脂肪酸的位置异构体和几何异构体。大多数脂肪酸的日内和日间CV为
Background: Targeted metabolomic analysis of fatty acids has linked the dysregulation of fatty acids to many diseases. This study selected five frequently used fatty acid derivatization methods for comparison.Methods: We compared the method precisions and derivatization efficiencies, the most economical and best performing method was subjected to method validation. Twenty-four fatty acid standards were used to validate the method, which was later applied to the investigation of potential fatty acid markers of breast cancer.Results: The acetyl chloride method was demonstrated to provide the best derivatization efficiency and lowest cost for plasma samples. The ionic liquid column successfully separated positional and geometric fatty acid isomers within 26 min under the optimized conditions. Intra-day and inter-day CVs for most of the fatty acids were