Fluid conductance of cancellous bone graft as a predictor for graft-host interface healing
Fluid conductance of cancellous bone graft as a predictor for graft-host interface healing
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DOI：
10.1016/0021-9290(95)00010-0
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发表时间：
1996-01-01
影响因子：
2.4
通讯作者：
Sher, A
中科院分区：
文献类型：
--
作者：
Hui, PW;Leung, PC;Sher, A
本研究的目的是验证松质骨移植物的流体传导性是移植物-宿主愈合的决定性因素这一假设。从新鲜猪股骨头上沿平行或垂直于股骨颈轴线的方向钻取圆柱形松质骨标本。将脱脂和冷冻干燥过程后的移植物置于灌注装置中。测量穿过骨移植物的压降和诱导流动，并将流体流动的传导率计算为流动-压力关系的斜率。将不同流导的移植物移植到35只家兔体内，以代替兔的胫骨段。移植后9周，处死29只完成随访且无事件的家兔。在移植物-宿主界面愈合期间，显微镜下检查移植物和相邻胫骨的脱钙切片的组织学事件。灌注数据表明，流体传导率范围为0.05 x 10（-10）至13.4 x 10（-10）m（3）s（-1）Pa-1，在平行于股骨颈轴的方向上较高，与移植物长度成反比，与孔隙率的平方成正比。家兔模型的数据支持上述假设。发现阈值电导为1.5 x 10（-10）m（3）s（-1）Pa-1，低于该值，不能实现血管重建以及成骨细胞和纤维组织的形成。本文简要讨论了低电导是如何导致移植物-宿主界面骨不连的。
The aim of this study is to test the hypothesis that the fluid conductance of cancellous bone graft is a deciding factor in graft-host union. Cylindrical cancellous bone specimens were trephined from fresh porcine femoral heads in a direction either parallel or perpendicular to the femoral neck axes. The graft after a defatting and freeze-drying process was placed in a perfusion apparatus. The pressure drop across the bone graft and the induced flow were measured and the conductance to fluid how was calculated as the slope of the how-pressure relation. Grafts of different flow conductance were transplanted into 35 rabbits to replace segments of tibiae. Nine weeks after grafting, 29 rabbits which had completed the follow-up without incidents were sacrificed. The decalcified sections of the grafts and the adjacent tibiae were examined microscopically for histological events during graft-host interface healing. Perfusion data indicated that fluid conductance ranged from 0.05 x 10(-10) to 13.4 x 10(-10) m(3) s(-1) Pa-1 and was higher in the direction parallel to the femoral neck axis, inversely proportional to the length of the graft and directly proportional to the square of porosity. Data from rabbit model supported the hypothesis stated above. A threshold conductance was found to be 1.5 x 10(-10) m(3) s(-1) Pa-1, below which revascularisation and the formations of osteoblasts and fibrous tissues could not be attained. How the low conductance led to non-union at the graft-host interface was briefly discussed.