Evolution of organoid technology: Lessons learnt in Co-Culture systems from developmental biology
Evolution of organoid technology: Lessons learnt in Co-Culture systems from developmental biology
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DOI：
10.1016/j.ydbio.2021.03.001
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发表时间：
2021-03-17
影响因子：
2.7
通讯作者：
Vosough, Massoud
中科院分区：
文献类型：
--
作者：
Zahmatkesh, Ensieh;Khoshdel-Rad, Niloofar;Vosough, Massoud
近年来，3D 类器官的发展为发育、生理学和再生医学的研究开辟了新的途径。类器官的形成和器官发生的过程具有共同的发育途径；因此，我们对发育生物学的了解可以帮助模拟不同器官的复杂性，从而将类器官细化为更复杂的平台。在胚胎发生过程中，发育过程强烈依赖于复杂的网络以及不同细胞群及其微环境之间细胞-细胞和细胞-基质相互作用的通讯。这些相互作用影响细胞行为，例如增殖、存活、迁移和分化。最近开发了类器官技术中的共培养系统，并提供了高度生理相关的系统。支持细胞包括各种类型的内皮细胞和基质细胞，提供适当的微环境，促进类器官组装，并改善类器官的血管化和成熟。本综述讨论了共培养系统在类器官生成中的作用，重点关注发育生物学知识如何指导并继续塑造更进化的 3D 共培养系统衍生的类器官的发展。
In recent years, the development of 3D organoids has opened new avenues of investigation into development, physiology, and regenerative medicine. Organoid formation and the process of organogenesis share common developmental pathways; thus, our knowledge of developmental biology can help model the complexity of different organs to refine organoids into a more sophisticated platform. The developmental process is strongly dependent on complex networks and communication of cell-cell and cell-matrix interactions among different cell populations and their microenvironment, during embryogenesis. These interactions affect cell behaviors such as proliferation, survival, migration, and differentiation. Co-culture systems within the organoid technology were recently developed and provided the highly physiologically relevant systems. Supportive cells including various types of endothelial and stromal cells provide the proper microenvironment, facilitate organoid assembly, and improve vascularization and maturation of organoids. This review discusses the role of the co-culture systems in organoid generation, with a focus on how knowledge of developmental biology has directed and continues to shape the development of more evolved 3D co-culture system-derived organoids.