Alginic Acid Oligosaccharide Suppresses Th2 Development and IgE Production by Inducing IL-12 Production
Alginic Acid Oligosaccharide Suppresses Th2 Development and IgE Production by Inducing IL-12 Production
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DOI：
10.1159/000076830
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发表时间：
2004-02
影响因子：
2.8
通讯作者：
Tadashi Yoshida;A. Hirano;H. Wada;Koji Takahashi;M. Hattori
中科院分区：
文献类型：
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作者：
Tadashi Yoshida;A. Hirano;H. Wada;Koji Takahashi;M. Hattori
背景资料：由于过敏原特异性IgE直接参与I型过敏反应，因此开发抑制导致IgE产生诱导的Th 2应答的方法将是预防过敏性疾病的有用方法。研究了从天然食用多糖中提取的海藻酸寡糖（alginic acid oligosaccharides，ALGO）对Th 2细胞的抑制作用及其机制。研究方法：在不存在或存在ALGO的情况下，将从β-乳球蛋白（β-LG）致敏的BALB/c小鼠获得的淋巴结细胞与抗原一起体外培养3天。测定各培养上清液中细胞因子的量。还通过使用卵清蛋白特异性T细胞受体转基因小鼠来检查ALGO对Th 2发育的影响。用β-LG或β-LG + ALGO免疫BALB/c小鼠，观察其血清抗体产生情况。结果：ALGO可剂量依赖性地上调抗原刺激诱导的IFN-γ的产生。ALGO还增强了IL-12的产生，并且向培养物中添加抗IL-12抗体消除了ALGO的作用。另一方面，在ALGO的存在下，由抗原刺激的转基因小鼠的脾细胞产生的IL-4被抑制。此外，与对照小鼠相比，ALGO处理的小鼠的IgE产生被显著抑制。结论：这些结果表明ALGO通过诱导IL-12的产生来抑制抗原诱导的Th 2发育。ALGO还抑制体内IgE的产生。这些结果表明，ALGO有望成为一种可食用的抗过敏剂。
Background: Since allergen-specific IgE is directly involved in the type I allergic reaction, development of a method for inhibiting Th2 responses which lead to the induction of IgE production would be a useful approach for preventing allergic disorders. The ability and mechanism of alginic acid oligosaccharide (ALGO), an oligosaccharide obtained from natural edible polysaccharide, for suppressing Th2 responses was examined in detail. Methods: Lymph node cells obtained from β-lactoglobulin (β-LG)-primed BALB/c mice were cultured in vitro with an antigen for 3 days in the absence or presence of ALGO. The amount of cytokine in each culture supernatant was measured. The effect of ALGO on Th2 development was also examined by using ovalbumin specific T cell receptor transgenic mice. Antibody production in the serum of BALB/c mice that had been immunized with β-LG or β-LG plus ALGO was investigated. Results: The production of IFN-γ induced by antigen stimulation was upregulated by ALGO in a dose-dependent manner. IL-12 production was also enhanced by ALGO, and the addition of the anti-IL-12 antibody to the culture abrogated the effect of ALGO. On the other hand, IL-4 production by antigen-stimulated splenocytes of transgenic mice was suppressed in the presence of ALGO. Furthermore, IgE production by ALGO-treated mice was significantly inhibited compared with control mice. Conclusions: These results indicate that ALGO suppressed antigen-induced Th2 development by inducing IL-12 production. ALGO also inhibited in vivo IgE production. These findings suggest that ALGO is expected to be an edible anti-allergic agent.