Neural cell adhesion molecule, neuron-specific enolase and calcitonin gene-related peptide immunoreactivity in hamster taste buds after chorda tympani/lingual nerve denervation.
Neural cell adhesion molecule, neuron-specific enolase and calcitonin gene-related peptide immunoreactivity in hamster taste buds after chorda tympani/lingual nerve denervation.
复制标题
鼓索/舌神经去神经支配后仓鼠味蕾中神经细胞粘附分子、神经元特异性烯醇化酶和降钙素基因相关肽的免疫反应性。
DOI：
10.1016/s0306-4522(97)00442-9
复制
发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Yao,B
中科院分区：
文献类型：
--
作者：
Whitehead,MC;Ganchrow,JR;Ganchrow,D;Yao,B
仓鼠蕈状乳头味蕾在感觉神经丧失后以萎缩形式存在。虽然萎缩味蕾和受神经支配的味蕾在形态上是相似的，但尚不清楚它们的生殖细胞是否具有相似的分子特征。神经细胞粘附分子、神经元特异性烯醇化酶和降钙素基因相关肽三种神经化学物质参与神经元和感觉神经上皮的营养现象、突触发生和细胞识别。本研究使用这些分子标记的免疫细胞化学定位来表征仓鼠单侧鼓室索/舌神经去神经后正常和去神经的真菌样味蕾。在正常味蕾中，对神经细胞粘附分子、神经元特异性烯醇化酶和降钙素基因相关肽的免疫反应存在于位于芽中心的一组细胞以及真菌状神经纤维和末梢中。去神经支配后，生殖细胞对所有三种标记物的免疫反应性降低，通常局限于单个或几个芽细胞。此外，除了血管和真菌状乳头内稀疏的降钙素基因相关肽免疫反应纤维外，未见纤维染色。这些剩余的纤维可能是自主纤维或躯体运动纤维。这些结果表明，仓鼠味蕾的感觉去神经支配降低了存活的生殖细胞对神经细胞粘附分子、神经元特异性烯醇酶和降钙素基因相关肽的免疫反应性，但并未完全消除。虽然表达这些标记物的味蕾细胞数量似乎是神经依赖性的，但仓鼠感觉失神经味蕾细胞的免疫反应性可能反映了局部组织因素的影响。
Hamster fungiform papilla taste buds persist in an atrophic form following sensory denervation. While atrophic and innervated taste buds are morphologically similar, it is not known whether their gemmal cells have similar molecular characteristics. Three neurochemicals, neural cell adhesion molecule, neuron-specific enolase, and calcitonin gene-related peptide have been implicated in trophic phenomena, synaptogenesis and cell recognition in neurons and sensory neuroepithelia. The present study uses immunocytochemical localization of these molecular markers to characterize normal and denervated fungiform taste buds following unilateral chorda tympani/lingual nerve denervation in hamsters. In normal taste buds, immunoreactivity to neural cell adhesion molecule, neuron-specific enolase, and calcitonin gene-related peptide was present in a group of cells located centrally in the bud as well as in fungiform nerve fibres and endings. After denervation, gemmal cell immunoreactivity to all three markers was reduced and often confined to a single or a few bud cell(s). Also, fibre staining was absent except for sparse calcitonin gene-related peptide-immunoreactive fibres associated with blood vessels and within the fungiform papillae. These remaining fibres may be autonomic or somatomotor in origin. These results indicate that sensory denervation of hamster taste buds reduces, but does not wholly eliminate the immunoreactivity of surviving gemmal cells to neural cell adhesion molecule, neuron-specific enolase, and calcitonin gene-related peptide. While the number of taste bud cells expressing the markers appears to be nerve-dependent, immunoreactivity in sensory-denervated bud cells of hamster may reflect the influence of local tissue factors.