EMR1, the human homolog of F4/80, is an eosinophil-specific receptor
EMR1, the human homolog of F4/80, is an eosinophil-specific receptor
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DOI：
10.1002/eji.200737553
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发表时间：
2007-10-01
影响因子：
5.4
通讯作者：
Kwakkenbos, Mark J.
中科院分区：
文献类型：
--
作者：
Hamann, Jorg;Koning, Nathalie;Kwakkenbos, Mark J.
EGF - TM7 F4/80是小鼠巨噬细胞群的一个确定标志物。通过使用新产生的单克隆抗体A10进行流式细胞术分析以及实时定量PCR，我们在此报告一个令人惊讶的发现：F4/80的人类同源物，即含表皮生长因子样结构域的粘蛋白样激素受体（EMR）1，在单核吞噬细胞包括单核细胞、巨噬细胞和髓样树突状细胞上缺失。出乎意料的是，我们发现EMR1的表达仅限于嗜酸性粒细胞，其表达与嗜酸性粒细胞趋化因子受体CCR3和免疫球蛋白样凝集素Siglec - 8重叠。在包括嗜碱性粒细胞在内的其他白细胞上缺失，意味着EMR1是人类嗜酸性粒细胞的一种高度特异性标志物。
The EGF-TM7 F4/80 is a defining marker of murine macrophage populations. Applying flow cytometric analysis using the newly generated mAb A10, and quantitative real-time PCR, we here report the surprising observation that the human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells. Unexpectedly, we found that EMR1 expression is restricted to eosinophilic granulocytes, where expression is overlapping with the eotaxin receptor CCR3 and the immunoglobulin-like lectin Siglec-8. Absence on other leukocytes, including basophils, implies that EMR1 is a highly specific marker for eosinophils in humans.