Bone marrow mononuclear cells and mannose receptor expression in focal cortical ischemia
Bone marrow mononuclear cells and mannose receptor expression in focal cortical ischemia
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DOI：
10.1016/j.brainres.2012.03.002
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发表时间：
2012-05-03
期刊：
影响因子：
2.9
通讯作者：
Baetas-da-Cruz, Wagner
中科院分区：
文献类型：
--
作者：
Giraldi-Guimaraes, Arthur;de Freitas, Helder Teixeira;Baetas-da-Cruz, Wagner
使用骨髓单个核细胞（BMMC）已被证明是一种公认的有效治疗中风的方法。然而，治疗作用的机制还不完全清楚。甘露糖受体（MR）是C型凝集素受体超家族的一个亚群，参与多种组织的先天性免疫应答。虽然主要以其免疫功能而闻名，但MR在炎症和伤口愈合期间的细胞迁移，细胞碎片清除和组织重塑中也具有重要作用。在这里，我们分析了MR表达的大鼠大脑中诱导单侧局灶性皮层缺血后一周的感觉运动皮层血管热凝。此外，我们评估了缺血加骨髓基质细胞治疗的大鼠皮质中这种表达的可能变化。我们的研究结果表明，在一个未知的GFAP（+）细胞类型和吞噬巨噬细胞/小胶质细胞在病变边界区的MR高表达，而在非损伤（对侧）皮质实质，MR表达水平低。此外，骨髓基质细胞治疗诱导MR在同侧（损伤）皮质的过度表达。我们小组以前的研究表明，在相同的局灶性皮质缺血模型中，BMMC治疗的大鼠功能恢复和神经退行性变减少。因此，我们认为缺血性损伤诱导MR表达的大量增加是清除损伤相关分子模式（DAMP）机制的一部分。此外，诱导骨髓基质细胞的MR过表达可能会增加清除效率，这些细胞的保护机制之一。(C)2012爱思唯尔有限公司版权所有。
The use of bone marrow mononuclear cells (BMMCs) has been shown as a putative efficient therapy for stroke. However, the mechanisms of therapeutic action are not yet completely known. Mannose receptor (MR) is a subgroup of the C-type lectin receptor superfamily involved in innate immune response in several tissues. Although known primarily for its immune function, MR also has important roles in cell migration, cell debris clearance and tissue remodeling during inflammation and wound healing. Here we analyzed MR expression in brains of rats one week after induction of unilateral focal cortical ischemia by thermocoagulation in blood vessels of sensorimotor cortex. Additionally, we evaluated possible changes in such expression in cortices of rats subjected to ischemia plus treatment with BMMCs. Our results showed high expression of MR in an unknown GFAP(+) cell type and in phagocytic macrophages/microglia within the lesion boundary zone whereas in the non-injured (contralateral) cortical parenchyma, low levels of MR expression were observed. Moreover, therapy with BMMCs induced overexpression of MR in ipsilateral (injured) cortex. Previous studies from our group have shown functional recovery and decreased neurodegeneration in BMMC-treated rats in the same model of focal cortical ischemia. Thus, we suggest that ischemic injury induces large increase in MR expression as part of a mechanism for clearance of damage-associated molecular patterns (DAMPs). In addition, induction of MR overexpression by BMMCs might increase the efficiency of clearance, being one of the protective mechanisms of these cells. (C) 2012 Elsevier B.V. All rights reserved.