IMMUNIZATION OF MICE BY RECOMBINANT OSPA PREPARATIONS AND PROTECTION AGAINST BORRELIA-BURGDORFERI INFECTION-INDUCED BY IXODES-RICINUS TICK BITES
IMMUNIZATION OF MICE BY RECOMBINANT OSPA PREPARATIONS AND PROTECTION AGAINST BORRELIA-BURGDORFERI INFECTION-INDUCED BY IXODES-RICINUS TICK BITES
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DOI：
10.1016/0165-2478(94)90166-x
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发表时间：
1994-03-01
影响因子：
4.4
通讯作者：
PETRE, J
中科院分区：
文献类型：
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作者：
GERN, L;RAIS, O;PETRE, J
伯氏疏螺旋体（Borreliaburgdorferi）是引起莱姆病的螺旋体，其广泛分布代表了世界许多地区的人类健康危害。疫苗接种被认为是一种有效的预防策略。接种实验用源自伯氏疏螺旋体菌株107的重组外表面蛋白A（OspA）的制剂进行。小鼠接受三剂（每剂1 μ g）吸附在氢氧化铝上的抗原。观察到对疫苗抗原的强烈免疫应答。在免疫后，使用感染伯氏疏螺旋体菌株107的蓖麻硬蜱若虫蜱攻击小鼠。通过耳活检培养、未感染幼虫的异种诊断和对伯氏疏螺旋体抗原的血清学反应来调查感染。发现所有未免疫的对照动物都被感染，而所有免疫的动物都被保护免受伯氏疏螺旋体感染。此外，发现大多数来自以免疫小鼠为食的蜱虫的成年蜱不含螺旋体。
The wide distribution of Borrelia burgdorferi, the spirochete causing Lyme borreliosis, represents a human health hazard in many areas of the world. Vaccination has been proposed as an effective prevention strategy. Vaccination experiments were conducted with preparations of recombinant outer surface protein A (OspA) derived from Borrelia burgdorferi strain ZS7. Mice received three doses (1 mu g each) of the antigens adsorbed to aluminum hydroxide. A strong immune response to the vaccine antigen was observed. Mice were challenged after immunization, using Ixodes ricinus nymphal ticks infected with Borrelia burgdorferi strain ZS7. Infection was investigated by ear biopsy culture, xenodiagnosis with uninfected larvae and serological response to Borrelia burgdorferi antigens. All unimmunized control animals were found to be infected, while all immunized animals were found to be protected against infection by Borrelia burgdorferi. In addition, most adult ticks derived from nymphs that fed on immunized mice were found to be free of spirochetes.