Effects of colonisation by different strains of Coniothyrium minitans on the viability of sclerotia of Sclerotinia sclerotiorum
Effects of colonisation by different strains of Coniothyrium minitans on the viability of sclerotia of Sclerotinia sclerotiorum
复制标题
不同菌株Coniothyrium minitans定植对核盘菌菌核活力的影响
DOI：
10.1080/09583157.2014.989386
复制
发表时间：
2015-01-01
影响因子：
1.4
通讯作者：
Yang, Xiaobing
中科院分区：
文献类型：
--
作者：
Jing, Lan;Navi, Shrishail S.;Yang, Xiaobing
白色霉菌是大豆生产中的主要病害。生物杀菌剂的使用是降低油菜菌核病危害的有效措施。对盾壳霉进行了菌株分离和对S. sclerotiorum的研究。在一系列实验中比较了来自美国爱荷华州的菌株N 09（GenBank登录号HQ 908274）和Contans® WG的菌株CON/M/91-08的分生孢子喷雾的功效。与CON/M/91-08和对照相比，在3°C下75天和90天取样，用N 09喷洒的菌核侵染灭菌土壤（SISS）和菌核侵染未灭菌土壤（SIUS）中的菌核存活率显著（P < 0.05）较低。类似地，与CON/M/91-08和对照相比，在23°C下，SISS中45、60和75天取样以及SIUS中45、75和90天取样的菌核活力显著（P < 0.05）较低。相比之下，N 09集落的存活率在整个采样期内在SISS中在3°C和23°C下均显著（P < 0.05）高于CON/M/91-08。而在SIUS中，N 09菌落在3°C下15、30、45、75和90 d采样时显著较高，在23°C下30、60和75 d采样时显著较高。此外，（1）N 09具有更快的生长速率，并且产生比CON/M/91-08多1.5倍的分生孢子;（2）N 09的真菌寄生比CON/M/91-08快;以及（3）菌核和菌株的共接种，N 09显示出比CON/M/91-08低的菌核繁殖。我们的数据表明，新菌株N 09在杀死菌核方面比CON/M/91-08具有更高的效率。
White mold is a major disease in commercial soybean production. An effective measure to reduce the negative effects of Sclerotinia sclerotiorum is the use of bio-fungicides. Strains of Coniothyrium minitans were isolated and efficacy tests against S. sclerotiorum was studied. The efficacy of pycnidiospores sprays of strain N09 (GenBank Accession No HQ908274) from Iowa, USA and strain CON/M/91-08 of Contans® WG were compared in a series of experiments. Sclerotia viability was significantly (P < 0.05) lower in both sclerotia-infested-sterilized-soils (SISS) and sclerotia-infested-unsterilized-soils (SIUS) sprayed with N09 compared with CON/M/91-08 and control at 3°C for 75d and 90d sampling. Similarly, sclerotia viability was significantly (P < 0.05) lower at 23°C for 45, 60 and 75d sampling in SISS and 45, 75 and 90 d sampling in SIUS compared with CON/M/91-08 and control. In contrast, viability of N09 colonies were significantly (P < 0.05) higher than that of CON/M/91-08 both at 3°C and 23°C in SISS across sampling periods. While in SIUS, N09 colonies were significantly higher at 3°C for 15, 30, 45, 75 and 90 d sampling, and at 23°C for 30, 60 and 75 d sampling. Also, (1) N09 had a faster growth rate and produced 1.5 times more pycnidiospores than CON/M/91-08; (2) mycoparasitism by N09 was faster than CON/M/91-08; and (3) co-inoculation of sclerotia and the strains, N09 showed lower sclerotia reproduction than CON/M/91-08. Our data suggest that the new strain N09 has a greater efficiency than CON/M/91-08 in killing sclerotia.