Human neural stem cell transplantation promotes functional recovery in rats with experimental intracerebral hemorrhage
Human neural stem cell transplantation promotes functional recovery in rats with experimental intracerebral hemorrhage
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DOI：
10.1161/01.str.0000083698.20199.1f
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发表时间：
2003-09-01
期刊：
影响因子：
8.3
通讯作者：
Roh, JK
中科院分区：
文献类型：
--
作者：
Jeong, SW;Chu, K;Roh, JK
背景和目的-细胞移植已被用于减少脑缺血的行为缺陷。然而，细胞移植治疗实验性脑出血（ICH）尚未见报道。我们假设，静脉内移植的人神经干细胞（NSC）可以迁移和分化成神经元或神经胶质细胞，从而提高功能的结果在ICH. Methods实验性ICH诱导的纹状体内注射细菌胶原酶在成年大鼠。手术后一天，将大鼠随机分为2组，静脉内接受永生化Lac z阳性人NSC（500穆尔中5 × 10（6）个细胞，n=12）或等量的盐水（n=13）。用改良的肢体放置和旋转棒测试对动物进行8周的评价。结果脑内移植的神经干细胞选择性迁移至血肿周围，并分化为神经元（约10%的β-gal（+）细胞）和星形胶质细胞（约75%）。神经干细胞移植组2周后转棒试验和5周后改良肢体放置试验的功能表现均优于对照组（P
Background and Purpose-Cell transplantation has been used to reduce behavioral deficit in cerebral ischemia. However, there is no report about cell transplantation in experimental intracerebral hemorrhage (ICH). We hypothesize that intravenously transplanted human neural stem cells (NSCs) can migrate and differentiate into neurons or glial cells, thereby improving functional outcome in ICH.Methods-Experimental ICH was induced by intrastriatal administration of bacterial collagenase in adult rats. One day after surgery, the rats were randomly divided into 2 groups to receive intravenously either immortalized Lac z-positive human NSCs (5x10(6) cells in 500 muL, n=12) or the same amount of saline (n=13). The animals were evaluated for 8 weeks with modified limb placing and rotarod tests. Transplanted NSCs were detected by X-gal histochemistry or beta-gal immunohistochemistry with double labeling of GFAP, NeuN, neurofilament, or CNPase.Results-Intravenously transplanted NSCs migrated selectively to the perihematomal areas and differentiated into neurons (approximate to10% of beta-gal(+) cells) and astrocytes (approximate to75%). The NSC-transplanted group showed better functional performance on rotarod test after 2 weeks and on modified limb placing test after 5 weeks compared with the control group (P