A Novel Kinesin 13 Protein Regulating Rice Seed Length
A Novel Kinesin 13 Protein Regulating Rice Seed Length
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DOI：
10.1093/pcp/pcq092
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发表时间：
2010-08-01
影响因子：
4.9
通讯作者：
Iwasaki, Yukimoto
中科院分区：
文献类型：
--
作者：
Kitagawa, Kanako;Kurinami, Shigeru;Iwasaki, Yukimoto
利用图位克隆技术，从水稻中克隆到一个新的小而圆的种子突变表型（srs3）的致病基因，命名为srs3基因。SRS3基因被归类为驱动蛋白13亚家族的成员。SRS3基因编码819个氨基酸的蛋白质，其包含驱动蛋白马达结构域和卷曲螺旋结构。利用扫描电子显微镜，我们确定了srs3中种子在纵向方向上的细胞长度比野生型短。srs3种子纵向细胞数与野生型差异不大。结果表明，srs3的小而圆的种子表型是由于种子的细胞长度在纵向上缩短。在粗微粒体组分中发现的SRS3蛋白在发育器官中高度表达。
The causal gene of a novel small and round seed mutant phenotype (srs3) in rice was identified by map-based cloning and named the SRS3 gene. The SRS3 gene was grouped as a member of the kinesin 13 subfamily. The SRS3 gene codes for a protein of 819 amino acids that contains a kinesin motor domain and a coiled-coil structure. Using scanning electron microscopy, we determined that the cell length of seeds in the longitudinal direction in srs3 is shorter than that in the wild type. The number of cells of seeds in the longitudinal direction in srs3 was not very different from that in the wild type. The result suggests that the small and round seed phenotype of srs3 is due to a reduction in cell length of seeds in the longitudinal direction. The SRS3 protein, which is found in the crude microsomal fraction, is highly expressed in developing organs.