Improvement stability and performance of invertase via immobilization on to silanized and polymer brush grafted magnetic nanoparticles
Improvement stability and performance of invertase via immobilization on to silanized and polymer brush grafted magnetic nanoparticles
复制标题
DOI：
10.1016/j.foodchem.2016.11.007
复制
发表时间：
2017-04-15
期刊：
影响因子：
8.8
通讯作者：
Arica, M. Yakup
中科院分区：
文献类型：
--
作者：
Bayramoglu, Gulay;Doz, Tugce;Arica, M. Yakup
在本研究中，磁性纳米粒子（Fe3O4）依次用二氧化硅（Fe3O4@SiO2）、甲基丙烯酸缩水甘油酯（GMA）通过表面引发原子转移自由基聚合（SI-ATRP）和六亚甲基二胺（作为间隔臂）进行修饰。 p(GMA)接枝和SA修饰形式（即Fe3O4@SiO2@pGMA-SA-3）用于共价固定转化酶（EC 3.2.1.26）。 Fe3O4@SiO2@p(GMA)和Fe3O4@SiO2@p(GMA)-SA-3上的固定化酶量分别为36.1+/-0.9和33.4+/-1.3mg/g。固定化转化酶的 K-m 和 V-max 值分别为 39.4 mmol/L 和 349.5 mmol/L min，与游离形式（34.3 mmol/L 和 387.2 mmol/L min）相比没有显着变化，表明所应用的方案对固定化转化酶的保留活性没有任何不利影响。 (C) 2016 Elsevier Ltd. 保留所有权利。
In this study, magnetic nanoparticles (Fe3O4) were modified sequentially with silica (Fe3O4@SiO2), glycidyl methacrylate (GMA) by surface initiated atom transfer radical polymerization (SI-ATRP) and hexamethylene diamine (as a spacer arm). The p(GMA) grafted and SA modified form (i.e., Fe3O4@SiO2@pGMA-SA-3) was used for covalent immobilization of invertase (EC 3.2.1.26). The amount of immobilized enzyme on Fe3O4@SiO2@p(GMA) and Fe3O4@SiO2@p(GMA)-SA-3 was 36.1 +/- 0.9 and 33.4 +/- 1.3 mg/g, respectively. The K-m and V-max values of immobilized invertase were found to be 39.4 mmol/L and 349.5 mmol/L min, and not significantly changed compared with free form (34.3 mmol/L and 387.2 mmol/L min), respectively, revealed that the applied protocol did not have any detrimental effect on the retained activity of immobilized invertase. (C) 2016 Elsevier Ltd. All rights reserved.