Microinjection of a cyclooxygenase inhibitor into the anteroventral preoptic region attenuates LPS fever
Microinjection of a cyclooxygenase inhibitor into the anteroventral preoptic region attenuates LPS fever
复制标题
DOI：
10.1152/ajpregu.1998.274.3.r783
复制
发表时间：
1998-03-01
影响因子：
2.8
通讯作者：
Saper, CB
中科院分区：
文献类型：
--
作者：
Scammell, TE;Griffin, JD;Saper, CB
相当多的证据支持前列腺素在发热中的作用，但引起发热的前列腺素合成的神经解剖学位点仍然未知。使用一种新的显微注射技术，我们将环氧合酶抑制剂酮咯酸注射到视前区（POA），以确定哪些视前区产生发热所需的三兰素。最初的实验表明，静脉注射酮咯酸阻断通常由脂多糖（LPS）5 μ g/kg iv引起的发热。微量注射酮咯酸到POA对体温没有影响，人工脑脊液注入POA没有改变LPS发热。注射酮咯酸到前腹POA显着降低了由LPS产生的发热，注射到更多的吻，尾，或背侧的位置相比。这些观察结果表明，前列腺素合成的前腹视前区是必要的生产发热。
Considerable evidence supports the role of prostaglandins in fever production, but the neuroanatomic sites of prostaglandin synthesis that produce fever remain unknown. With the use of a novel microinjection technique, we injected the cyclooxygenase inhibitor ketorolac into the preoptic area (POA) to determine which preoptic regions produce the prostaglandins required for fever. Initial experiments demonstrated that intravenous ketorolac blocked the fever normally produced by lipopolysaccharide (LPS) 5 mu g/kg iv. Microinjection of ketorolac into the POA had no effect on body temperature, and injection of artificial cerebrospinal fluid into the POA did not alter LPS fever. Injection of ketorolac into the anteroventral POA markedly decreased the fever produced by LPS, compared with injections into more rostral, caudal, or dorsal locations. These observations indicate that prostaglandin synthesis in the anteroventral preoptic region is necessary for the production of fever.