Hydrogen bond formation between the naturally modified nucleobase and phosphate backbone.
Hydrogen bond formation between the naturally modified nucleobase and phosphate backbone.
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DOI：
10.1093/nar/gks426
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发表时间：
2012-09
影响因子：
14.9
通讯作者：
Huang Z
中科院分区：
文献类型：
--
作者：
Sheng J;Zhang W;Hassan AE;Gan J;Soares AS;Geng S;Ren Y;Huang Z
Natural RNAs, especially tRNAs, are extensively modified to tailor structure and function diversities. Uracil is the most modified nucleobase among all natural nucleobases. Interestingly, >76% of uracil modifications are located on its 5-position. We have investigated the natural 5-methoxy (5-O-CH3) modification of uracil in the context of A-form oligonucleotide duplex. Our X-ray crystal structure indicates first a H-bond formation between the uracil 5-O-CH3 and its 5′-phosphate. This novel H-bond is not observed when the oxygen of 5-O-CH3 is replaced with a larger atom (selenium or sulfur). The 5-O-CH3 modification does not cause significant structure and stability alterations. Moreover, our computational study is consistent with the experimental observation. The investigation on the uracil 5-position demonstrates the importance of this RNA modification at the atomic level. Our finding suggests a general interaction between the nucleobase and backbone and reveals a plausible function of the tRNA 5-O-CH3 modification, which might potentially rigidify the local conformation and facilitates translation.
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DOI：
10.1107/s0907444995011115
发表时间：
1996-01-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Parkinson, G;Vojtechovsky, J;Berman, HM
通讯作者：
Berman, HM
影响因子：
15
作者：
EGERT, E;LINDNER, HJ;BOHM, MC
通讯作者：
BOHM, MC
DOI：
10.1107/s0108767386099622
发表时间：
1986-05-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION A
影响因子：
--
作者：
READ, RJ
通讯作者：
READ, RJ
影响因子：
15
作者：
Hassan, Abdalla E. A.;Sheng, Jia;Huang, Zhen
通讯作者：
Huang, Zhen
影响因子：
4.4
作者：
BECKE, AD
通讯作者：
BECKE, AD