Neuroendocrine immune-regulatory of a neuropeptide ChGnRH from the Hongkong oyster, Crassostrea Hongkongensis
Neuroendocrine immune-regulatory of a neuropeptide ChGnRH from the Hongkong oyster, Crassostrea Hongkongensis
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香港牡蛎神经肽 ChGnRH 的神经内分泌免疫调节作用
DOI：
10.1016/j.fsi.2019.05.055
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发表时间：
2019
影响因子：
4.7
通讯作者：
Chen Dongbo
中科院分区：
文献类型：
--
作者：
Huang Qingsong;Li Qiuhong;Chen Hongmei;Lin Baohua;Chen Dongbo
人们越来越认识到，神经内分泌-免疫相互作用是理解复杂免疫系统的关键。在这项研究中，我们探讨了与生殖调节相关的激素GnRH在香港牡蛎免疫调节中的作用。我们发现，弧菌菌株注射后，ChGnRH的表达增加。此外，ChGnRH神经肽还能促进作为中枢免疫器官的血细胞的吞噬能力和细菌清除作用。与ChGnRH多肽孵育后，cAMP含量增加，可被腺酰环化酶抑制剂SQ 22,536阻断。此外，SQ 22、536、H89和enzastaurin也阻断了ChGnRH多肽对巨噬细胞吞噬和细菌清除的刺激作用，有力地表明cAMP依赖的PKA和PKC信号通路参与了ChGnRH介导的免疫调节。综上所述，本研究证实了海洋无脊椎动物存在神经内分泌免疫调节系统，有助于理解牡蛎免疫防御系统的复杂性。
It is increasingly appreciated that neuroendocrine-immune interactions hold the key to understand the complex immune system. In this study, we explored the role of a reproductive regulation-related hormone, GnRH, in the regulation of immunity in Hong Kong oysters. We found that vibrio bacterial strains injection increased the expression ofChGnRH. Moreover,ChGnRH neuropeptide promotes the phagocytic ability and bacterial clearance effect of hemocytes which regarded to be the central immune organ. The content of cAMP after incubation withChGnRH peptide was increased, which could be blocked by adenylyl cyclase inhibitor SQ 22,536. Furthermore, the stimulated effect ofChGnRH peptide on the phagocytosis and bacterial clearance was also blocked by SQ 22,536, H89 and enzastaurin, strongly demonstrating that cAMP dependent PKA and PKC signaling pathway was involved inChGnRH mediated immune regulation. In conclusion, this study confirms the presence of neuroendocrine-immune regulatory system in marine invertebrates, which contributes to understand the complexity of oyster immune defense system.