Identification of genes related to mechanical stress in human periodontal ligament cells using microarray analysis
Identification of genes related to mechanical stress in human periodontal ligament cells using microarray analysis
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DOI：
10.1111/j.1600-0765.2006.00906.x
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发表时间：
2007-02-01
影响因子：
3.5
通讯作者：
Moriyama, K.
中科院分区：
文献类型：
--
作者：
de Araujo, R. M. S.;Oba, Y.;Moriyama, K.
背景与目的：人牙周膜(PDL)在机械应力(如正畸力)作用下基因的差异表达被认为与牙周膜细胞和牙周组织的重塑有关。然而，对于机械应力下PDL细胞的基因表达情况却知之甚少。材料与方法：我们采用基因芯片技术，利用体外三维培养系统，对静力作用下PDL细胞的基因表达谱进行了全面的研究。选择6个基因进行实时定量聚合酶链式反应分析，验证结果与芯片数据一致。结果：芯片数据显示30,000个差异表达基因中有108个是机械力加载差异表达的。其中，85个基因在机械应激下上调，23个基因在机械应激下表达下调，以与对照组相比增加/减少两倍的阈值来判断。其中32个上调基因和8个下调基因参与了多种生物学过程，包括细胞通讯、细胞信号、细胞周期、应激反应和钙释放等。然而，在我们的微阵列数据中，有几个差异表达的基因还没有被很好地定义为应激反应分子。结论：我们的微阵列是第一个展示机械应力导致的PDL细胞中的基因图谱，但这些分子在PDL细胞中的生理功能还需要进一步的研究。
Background and Objective: Differential expression of genes in human periodontal ligament (PDL) under mechanical stress, such as orthodontic force, is thought to be involved in the remodeling of PDL cells and periodontal tissues. However, little is known about the genes expressed in PDL cells under mechanical stress.Material and Methods: We employed microarray analysis to assess, in a comprehensive manner, the gene expression profiles in PDL cells compressed by a static force using an in vitro three-dimensional culture system. Six genes were selected and validated by quantitative real-time polymerase chain reaction analysis, consistent with the microarray data.Results: The microarray data revealed that 108 of 30,000 genes tested were differentially expressed by mechanical force loading. Among them, 85 genes were up-regulated by mechanical stress, while 23 genes were down-regulated, judging by the thresholds of a two-fold increase/decrease compared with the controls. Thirty-two of the up-regulated and eight of the down-regulated genes, well-characterized in protein function, were involved in numerous biological processes including cell communication, cell signaling, cell cycle, stress response, and calcium release. However, several genes differentially expressed in our microarray data have not been well defined as stress-response molecules.Conclusion: Our microarray is the first to show the gene profile in PDL cells caused by mechanical stress; however, further studies to clarify the physiological function of these molecules in PDL cells are required.