A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity
A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity
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DOI：
10.1089/vim.2009.0090
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发表时间：
2010-04-01
期刊：
影响因子：
2.2
通讯作者：
Jiang, Shibo
中科院分区：
文献类型：
--
作者：
Du, Lanying;Zhao, Guangyu;Jiang, Shibo
严重急性呼吸道综合征（SARS）是由SARS冠状病毒（SARS-CoV）引起的一种新型传染病，其疫苗的研制是预防SARS复发的关键。SARS-CoV的刺突蛋白（S），尤其是受体结合域（RBD）在预防SARS感染中起重要作用，是SARS疫苗研究的重要组成部分。在这项研究中，我们在中国仓鼠卵巢（CHO）-K1细胞（RBD 219-CHO）中表达了219-mer（残基318-536）RBD蛋白，并在小鼠模型中检测了其免疫应答和保护性免疫。结果表明，重组蛋白折叠正确，能够保持完整的构象和真实的抗原性。免疫小鼠可产生较强的体液免疫和细胞免疫应答，并产生高滴度的中和抗体。RBD 219-CHO蛋白诱导了有效的保护性免疫，保护所有接种小鼠免受SARS-CoV攻击。这些结果表明，重组RBD 219-CHO蛋白具有很大的潜力，为开发一种有效的和安全的SARS亚单位疫苗。
Development of vaccines is essential for the prevention of future recurrences of severe acute respiratory syndrome (SARS), caused by the SARS coronavirus (SARS-CoV). The spike (S) protein, especially receptorbinding domain (RBD) of SARS-CoV, plays important roles in the prevention of SARS infection, and is thus an important component in SARS vaccine development. In this study, we expressed a 219-mer (residues 318-536) RBD protein in Chinese hamster ovary (CHO)-K1 cells (RBD219-CHO), and tested its immune responses and protective immunity in a mouse model. The results showed that this recombinant protein was correctly folded, being able to maintain intact conformation and authentic antigenicity. It could induce strong humoral and cellular immune responses and high titers of neutralizing antibodies in the vaccinated mice. RBD219-CHO protein elicited potent protective immunity that protected all vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD219-CHO protein has great potential for the development of an effective and safe SARS subunit vaccine.