Effect of Lactobacillus pentosus ONRIC b0240 on intestinal IgA production in mice fed differing levels of protein.
Effect of Lactobacillus pentosus ONRIC b0240 on intestinal IgA production in mice fed differing levels of protein.
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DOI：
10.1021/jf104240d
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发表时间：
2011-02
影响因子：
6.1
通讯作者：
T. Shimosato;Kazuaki Tomida;H. Otani
中科院分区：
文献类型：
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作者：
T. Shimosato;Kazuaki Tomida;H. Otani
蛋白质-能量营养不良（PEM）通常与免疫功能下降引起的感染有关。在此，通过在PEM模型小鼠中施用戊糖乳杆菌ONRIC b 0240（b240）来进行以增强粘膜免疫为目的的实验。将三组约12周龄的雄性C3 H/HeN小鼠以五或六只为一组关进笼子，并接受各种治疗。给小鼠喂食4（低蛋白饮食; PEM模型）、20（标准蛋白饮食）或40%（高蛋白饮食）的卵清蛋白（OVA），含或不含0.05%b240。五周后，处死所有小鼠，并提取器官用于分析免疫应答。本研究未观察到急性毒性。添加b240对体重没有影响;然而，体重随着蛋白质水平的增加而下降。有趣的是，在含有b240的所有试验饮食中，肠总伊加显著增加（p<0.05）。体外实验表明，经b240处理的小鼠脾细胞中B细胞和2型辅助性T（Th 2）细胞数量显著增加，而Th 1细胞数量无明显差异。b240还具有增加小鼠派伊尔斑细胞中伊加和IgG产生的能力。这些结果表明，b240通过增强体液免疫增强PEM模型小鼠的伊加产生并帮助恢复肠道免疫系统。
Protein-energy malnutrition (PEM) is frequently associated with the occurrence of infection due to a decline in immune function. Here, an experiment was conducted with the objective of enhancing mucosal immunity by administration of Lactobacillus pentosus ONRIC b0240 (b240) in PEM model mice. Three groups of male C3H/HeN mice aged approximately 12 weeks were caged in groups of five or six and received various treatments. The mice were fed 4 (low-protein diet; PEM model), 20 (standard-protein diet), or 40% (high-protein diet) ovalbumin (OVA) with or without 0.05% b240. Five weeks later, all mice were sacrificed, and the organs were extracted for analysis of the immune response. Acute toxicity was not observed in this study. The addition of b240 showed no influence on body weight; however, body weight decreased with increasing protein level. Interestingly, intestinal total IgA was significantly increased (p<0.05) in all test diets with b240. The in vitro study showed that the number of B cells and type 2 helper T (Th2) cells were significantly increased in mouse spleen cells with b240 treatment, whereas no differences were found in the number of Th1 cells. b240 also has the ability to augment IgA and IgG production in mouse Peyer's patch cells. These results suggest that b240 enhances IgA production and helps recover the intestinal immune system in PEM model mice via augmentation of humoral immunity.