Two mechanisms for increasing milt volume in male goldfish,Carassius auratus
Two mechanisms for increasing milt volume in male goldfish,Carassius auratus
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增加雄性金鱼精液体积的两种机制
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
N. Stacey
中科院分区：
文献类型：
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作者：
Wenbin Zheng;N. Stacey
金鱼（Carassius auratus）在与雌性注射前列腺素f2 α（产卵刺激）和甾体信息素17α，20β-二羟基-4-孕-3-one （17,20β- p）的产卵相互作用下，其卵黄体积增加。在这项研究中，我们通过决定milt反应的潜伏期，以及比较水温、垂体切除术和人绒毛膜促性腺激素（hCG）注射对milt反应的影响，寻找产卵刺激和1720 β- p通过不同机制增加milt体积的证据。在21°C和11°C条件下，产卵刺激均能在2小时内增加产蛋量，而17、20β-P暴露或hCG注射直到21°C条件下4-6小时和11°C条件下12-24小时才会增加产蛋量。垂体切除术不能阻断产卵诱导的milt增加，但可以阻断17,20β- p诱导的milt增加，这与早期研究表明信息素17,20β- p通过增加血液促性腺激素ii （GtH-II）水平起作用一致。此外，在注射hCG以消除17,20β- p诱导的GtH-II增加的影响的雄性中，产卵刺激引起的milt增加，而暴露于17,20β- p则没有。这些结果表明，不同的机制介导了产卵刺激和17,20β- p对金鱼乳体积的影响。17,20β- p明显通过释放垂体GtH-II起作用，但其介导产卵刺激作用的机制尚不清楚。讨论了这两种机制在生殖功能中的作用。©1996 Wiley-Liss, Inc
Milt volume in goldfish (Carassius auratus) is known to increase in response to both spawning interactions with prostaglandin F2α-injected females (spawning stimuli) and a steroidal pheromone, 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P). In this study, we sought evidence that spawning stimuli and 17,20β-P increase milt volume via different mechanisms, by determining latency of the milt response, and by comparing the effects of water temperature, hypophysectomy, and human chorionic gonadotropin (hCG) injection on milt response. At both 21 and 11°C, spawning stimuli increased milt volume within 2 hr, whereas 17,20β-P exposure or hCG injection did not increase milt volume until 4-6 hr at 21°C and 12-24 hr at 11°C. Hypophysectomy did not block spawning-induced milt increase but did block 17,20β-P-induced milt increase, consistent with earlier studies showing that pheromonal 17,20β-P acts by increasing blood gonadotropin-II (GtH-II) level. Furthermore, in males injected with hCG to swamp the effect of 17,20β-P-induced GtH-II increase, milt increase was observed in response to spawning stimuli but not to 17,20β-P exposure. These results indicate that distinct mechanisms mediate the effects of spawning stimuli and 17,20β-P on milt volume in goldfish. 17,20β-P evidently acts by releasing pituitary GtH-II, whereas the mechanism mediating the effect of spawning stimuli is unknown. The role of these two mechanisms in made reproductive function is discussed. © 1996 Wiley-Liss, Inc.