Microbial phenazine production enhances electron transfer in biofuel cells
Microbial phenazine production enhances electron transfer in biofuel cells
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DOI：
10.1021/es048563o
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发表时间：
2005-05-01
影响因子：
11.4
通讯作者：
Verstraete, W
中科院分区：
文献类型：
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作者：
Rabaey, K;Boon, N;Verstraete, W
在微生物燃料电池（MFC）中，向阳极的高速率电子转移迄今尚未被描述为细菌产生的可溶性氧化还原介体。为了研究电子转移的机制，我们使用MFC分离株，铜绿假单胞菌菌株KRP 1。通过绿脓菌素和吩嗪-1-甲酰胺使细菌电子向MFC阳极转移成为可能。阳极的存在刺激绿脓菌素的生产。缺乏绿脓菌素和吩嗪-1-甲酰胺合成的突变菌株无法实现实质性的电子转移，仅达到野生型功率输出的5%。添加绿脓菌素后，功率输出恢复至50%。绿脓菌素不仅被铜绿假单胞菌用于提高电子传递，而且也被其他细菌物种用于增强电子传递。一种细菌可以产生电子穿梭机，其他细菌也可以使用这种穿梭机来提高电子转移速率和生长，这一发现以前从未被证明过。这些研究结果具有相当大的影响，在MFC中可达到的功率输出。
High-rate electron transfer toward an anode in microbial fuel cells(MFCs) has thus far not been described for bacteria-producing soluble redox mediators. To study the mechanism of electron transfer, we used a MFC isolate, Pseudomonas aeruginosa strain KRP1. Bacterial electron transfer toward the MFC anode was enabled through pyocyanin and phenazine-l-carboxamide. The presence of the anode stimulated pyocyanin production. Mutant strains, deficient in the synthesis of pyocyanin and phenazine-1-carboxamide, were unable to achieve substantial electron transfer and reached only 5% of the wild type's power output. Upon pyocyanin addition, the power output was restored to 50%. Pyocyanin was not only used by P. aeruginosa to improve electron transfer but as well enhanced electron transfer by other bacterial species. The finding that one bacterium can produce electron shuttles, which can be used also by other bacteria, to enhance electron-transfer rate and growth, has not been shown before. These findings have considerable implications with respect to the power output attainable in MFCs.