Murine tracheal and nasal septal epithelium for air-liquid interface cultures: A comparative study
Murine tracheal and nasal septal epithelium for air-liquid interface cultures: A comparative study
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DOI：
10.2500/ajr.2007.21.3068
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发表时间：
2007-09-01
期刊：
影响因子：
--
通讯作者：
Cohen, Noam A.
中科院分区：
文献类型：
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作者：
Woodworth, Bradford A.;Antunes, Marcelo B.;Cohen, Noam A.
背景：使用小鼠气管呼吸道上皮细胞的气液界面培养已经彻底改变了气道疾病的体外研究。然而，这些培养通常是不切实际的，因为可以从小鼠气管分离的呼吸道上皮细胞的数量很少。体外研究纤毛生理学的能力在慢性鼻窦炎（CRS）的研究中至关重要。我们的假设是，小鼠鼻中隔是一个更好的纤毛呼吸上皮来源，建立呼吸上皮气液界面models.Methods：鼻中隔和气管从10只BALB/c小鼠。采用一种简单直接的新技术获取鼻中隔。对所有标本进行扫描电子显微镜检查。纤毛呼吸道上皮细胞的细胞计数在一个标准放大倍数（1535 x）下进行。比较分析近端和远端气管，中前和中后鼻中隔上皮，performed.Results：独立的细胞计数显示细胞群的比例非常显着差异（p < 0.00001）。气管纤毛细胞计数（106.9 +/- 28）平均占总细胞群的38.7%。鼻中隔纤毛上皮细胞（277.5 +/- 16）占总细胞数的90.1%.Conclusion：为提高小鼠呼吸道上皮细胞的产量，我们发现鼻中隔远比气管为上级来源。纤毛上皮细胞的更大表面积和增加的浓度有可能为气液界面培养的发展提供八倍的上皮细胞增加。
Background: Air-liquid interface cultures using murine tracheal respiratory epithelium have revolutionized the in vitro study of airway diseases. However, these cultures often are impractical because of the small number of respiratory epithelial cells that can be isolated from the mouse trachea. The ability to study ciliary physiology in vitro is of utmost importance in the research of chronic rhinosinusitis (CRS). Our hypothesis is that the murine nasal septum is a better source of ciliated respiratory epithelium to develop respiratory epithelial air-liquid interface models.Methods: Nasal septa and tracheas were harvested from 10 BALB/c mice. The nasal septa were harvested by using a simple and straightforward novel technique. Scanning electron microscopy was performed on all specimens. Cell counts of ciliated respiratory epithelial cells were performed at one standard magnification (1535x). Comparative analysis of proximal and distal trachea, midanterior and midposterior nasal septal epithelium, was performed.Results: Independent cell counts revealed highly significant differences in the proportion of cell populations (p < 0.00001). Ciliated cell counts for the trachea (106.9 +/- 28) were an average of 38.7% of the total cell population. Nasal septal ciliated epithelial cells (277.5 +/- 16) comprised 90.1% of the total cell population.Conclusion: To increase the yield of respiratory epithelial cells harvested from mice, we have found that the nasal septum is afar superior source when compared with the trachea. The greater surface area and increased concentration of ciliated epithelial cells has the potential to provide an eightfold increase in epithelial cells for the development of air-liquid interface cultures.