Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety
Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety
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大鼠嗅球的比较蛋白质组学揭示了对慢性压力引起的抑郁或焦虑的易感性和弹性的见解
DOI：
10.1016/j.neuroscience.2021.08.012
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发表时间：
2021-09-01
期刊：
影响因子：
3.3
通讯作者：
Zhou, Jian
中科院分区：
文献类型：
--
作者：
Liu, Dan;Cai, Xiao;Zhou, Jian
摘要压力可导致焦虑症和抑郁症患者嗅球异常，但其独特和共同的神经生物学机制尚不清楚。此前，我们按照慢性轻度压力（CMS）、抑郁易感（Dep-Sus）：具有抑郁样行为、焦虑易感（Anx-Sus）：具有焦虑样行为和不易感（Insus）：没有抑郁和焦虑样行为建立了三个组。为了进一步探讨这三组大鼠嗅球蛋白表达的变化，我们对嗅球进行了比较定量的蛋白质组学分析。接下来，实施生物信息学分析，而蛋白质表达通过平行反应监测（PRM）或蛋白质印迹（WB）独立地分析。OB蛋白质组分析共鉴定出133个差异表达蛋白质作为CMS反应。这些去调控的蛋白质参与多种功能和重要途径，可能与抑郁或焦虑的适应不良行为以及适应行为的表型相关，因此可能作为潜在的候选蛋白质靶点。随后基于PRM或WB的分析显示，Nefl、Mtmr 7和Tk 2; Prkaca、Coa 3、Cox 6c 2、Lamc 1和Tubal 3;以及Pabpn 1、Nme 3、Sos 1和Lum的变化分别与Dep-Sus、Anx-Sus和Insus组唯一相关。这些表型特异性去调节蛋白主要参与多种代谢和信号通路，这表明相同的CMS对嗅觉蛋白调节系统和生物过程有不同的影响。综上所述，我们目前的数据作为一个有用的蛋白质组学基础提供了共同的和独特的分子见解的生化理解OB功能障碍的潜在易感性和弹性慢性压力引起的焦虑或抑郁症。(c)2021年IBRO。由爱思唯尔有限公司出版。保留所有权利。
stress causes the abnormality of olfactory bulb (OB) in both anxiety and depression, however, the unique and common neurobiological underpinnings are still poorly understood. Previously, we built the three groups by chronic mild stress (CMS), depression-susceptible (Dep-Sus): with depression-like behavior, anxietysusceptible (Anx-Sus): with anxiety-like behavior and insusceptible (Insus): without depression- and anxiety-like behaviors. To continuously explore the protein expression changes in these three groups, comparative quantitative proteomics analysis was conducted on the rat OB as crucial part of the olfactory system. Next, bioinformatics analyses were implemented whereas protein expressions were independently analyzed by parallel reaction monitoring (PRM) or Western blot (WB). The OB-proteome analysis identified totally 133 differentially expressed proteins as a CMS response. These deregulated proteins were involved in multiple functions and significant pathways potentially correlated with phenotypes of maladaptive behavior of depression or anxiety as well as adaptive behavior, and hence might act as potential candidate protein targets. The subsequent PRM-based or WB-based analyses showed that changes in Nefl, Mtmr7 and Tk2; Prkaca, Coa3, Cox6c2, Lamc1 and Tubal3; and Pabpn1, Nme3, Sos1 and Lum were uniquely associated with Dep-Sus, Anx-Sus, and Insus groups, respectively. These phenotype-specific deregulated proteins were primarily involved in multiple metabolic and signaling pathways, suggesting that the identical CMS differently impacted the olfactory protein regulation system and biological processes. To sum up, our present data as a useful proteomics underpinning provided the common and distinct molecular insights into the biochemical understanding of OB dysfunction underlying susceptibility and resiliency to chronic-stress-induced anxiety or depression. (c) 2021 IBRO. Published by Elsevier Ltd. All rights reserved.