The effect of cigarette smoke on the permeability of guinea pig airways.
The effect of cigarette smoke on the permeability of guinea pig airways.
复制标题
香烟烟雾对豚鼠气道通透性的影响。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
J. Hogg
中科院分区：
文献类型：
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作者：
R. Boucher;J. Johnson;Sadayuki Inoue;W. Hulbert;J. Hogg
内衬传导气道的上皮限制吸入的异物进入间质和循环空间的运动。在本研究中，我们已经调查了分级剂量的吸入香烟烟雾对这一功能的影响，通过测量辣根过氧化物酶（HRP）从气道腔穿过气管上皮进入血液的运动速率，通过评估HRP进入气管上皮的渗透，使用透射电子显微镜，并通过检查使用冷冻断裂技术的气管上皮紧密连接的形态。这些研究表明，5、20和100喷全烟以剂量依赖性方式增加HRP从气管腔渗透到血液中。透射电子显微镜检查显示，HRP穿透气管上皮仅在100喷曝光组，而冷冻断裂研究显示，上皮紧密连接进行性破坏开始与100喷曝光，并成为更广泛的200喷曝光后。我们的结论是，吸入的香烟烟雾损害粘膜屏障，并导致增加通透性HRP破坏细胞间的紧密连接，并测量血液中的HRP的外观是更敏感的比电子显微镜在评估这种损害。
The epithelium lining the conducting airways restricts the movement of inhaled foreign materials into the interstitial and circulatory space. In the present study, we have investigated the effects of graded doses of inhaled whole cigarette smoke on this function by measuring the rate of movement of horseradish peroxidase (HRP) from the airways lumen across the tracheal epithelium into blood, by assessing the penetration of HRP into tracheal epithelium using transmission electron microscopy, and by examining the morphology of tracheal epithelial tight junctions using the freeze-fracture technique. These studies showed that 5, 20, and 100 puffs of whole smoke increased penetration of HRP from the tracheal lumen into blood in a dose-dependent fashion. Transmission electron microscopy showed that HRP penetrated the tracheal epithelium only in the 100-puff exposure group while the freeze-fracture studies showed progressive disruption of epithelial tight junction beginning with a 100-puff exposure and becoming more extensive after the 200-puff exposure. We conclude that inhaled cigarette smoke damages the mucosal barrier and causes increased permeability to HRP by disrupting the intercellular tight junctions and that measuring the appearance of HRP in blood is more sensitive than electron microscopy in assessing this damage.