Staphylocloccus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing
Staphylocloccus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing
复制标题
DOI：
10.1073/pnas.0404728101
复制
发表时间：
2004-08-17
影响因子：
11.1
通讯作者：
Missiakas, DM
中科院分区：
文献类型：
--
作者：
Bae, T;Banger, AK;Missiakas, DM
金黄色葡萄球菌是世界范围内伤口和医院获得性感染的主要原因。S.对多种抗生素具有抗性的金黄色葡萄球菌菌株需要鉴定细菌毒力基因和开发新的治疗策略。在此，使用基于水手的转座子囊进行随机突变并分离10，325个S。具有确定插入位点的金黄色葡萄球菌变体。通过在秀丽隐杆线虫杀伤试验中筛选功能丧失突变体，71 S。鉴定了金黄色葡萄球菌毒力基因。其中一些基因也是S.金黄色葡萄球菌脓肿形成的小鼠感染模型。
Staphylococcus aureus is the leading cause of wound and hospital-acquired infections worldwide. The emergence of S. aureus strains with resistance to multiple antibiotics requires the identification of bacterial virulence genes and the development of novel therapeutic strategies. Herein, bursa aurealis, a mariner-based transposon, was used for random mutagenesis and for the isolation of 10,325 S. aureus variants with defined insertion sites. By screening for loss-of-function mutants in a Caenorhabditis elegans killing assay, 71 S. aureus virulence genes were identified. Some of these genes are also required for S. aureus abscess formation in a murine infection model.