Usefulness of HBME-1, cytokeratin 19 and galectin-3 immunostaining in the diagnosis of thyroid malignancy
Usefulness of HBME-1, cytokeratin 19 and galectin-3 immunostaining in the diagnosis of thyroid malignancy
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DOI：
10.1111/j.1365-2559.2005.02221.x
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发表时间：
2005-10-01
期刊：
影响因子：
6.4
通讯作者：
Ward, LS
中科院分区：
文献类型：
--
作者：
de Matos, PS;Ferreira, AP;Ward, LS
目的：探讨一组甲状腺恶性肿瘤标志物（包括HBME-1、细胞角蛋白（CK） 19和半乳糖凝集素-3）免疫组织化学表达和免疫定位的有效性。方法和结果：我们评估了170例甲状腺病变，包括148例肿瘤性病变[84例乳头状癌（PC）， 38例滤泡癌（FC）， 18例滤泡腺瘤，1例透明化小梁瘤，5例髓样癌，2例间变性癌]和22例非肿瘤性病变（12例腺瘤结节和10例桥本甲状腺炎）。HBME-1、半乳糖凝集素-3和CK19在94%、72.6%、72.6%的pc和63%、21%、21%的fc中表达。三种标志物在所有正常组织中均为阴性。虽然在诊断PC滤泡变异型和FC的敏感性和特异性方面，最有用的标志物是HBME-1，但当我们考虑滤泡变异型乳头状癌（FVPC）和FC或腺瘤之间的差异时，就阳性细胞的百分比而言，半凝集素-3和CK19更相关。结论：HBME-1是甲状腺恶性肿瘤最敏感的标志物，但这三种标志物可能在特定病例中有用。这组标记物有助于区分滤泡型病变，特别是FVPC。
Aims: To investigate the usefulness of immunohistochemical expression and immunolocalization of a panel of thyroid malignancy markers including HBME-1, cytokeratin (CK) 19 and galectin-3.Methods and results: We evaluated 170 thyroid lesions including 148 neoplastic lesions [84 papillary carcinomas (PC), 38 follicular carcinomas (FC), 18 follicular adenomas, one hyalinizing trabecular tumour, five medullary carcinomas, two anaplastic carcinomas] and 22 non-neoplastic lesions (12 adenomatous nodules and 10 Hashimoto's thyroiditis). HBME-1, galectin-3 and CK19 were expressed in 94%, 72.6%, 72.6% of PCs and in 63%, 21%, 21% of FCs. The three markers were mostly negative in all normal tissues. Although the most helpful marker in terms of sensitivity and specificity for the follicular variant of PC and for FC diagnosis was HBME-1, when we consider the differentiation between cases of follicular variant of papillary carcinoma (FVPC) and FC or adenoma, in terms of percentage of positive cells, galectin-3 and CK19 were more relevant.Conclusions: HBME-1 is the most sensitive marker for thyroid malignancy but the three markers may be useful in specific cases. This panel of markers is useful to differentiate the follicular patterned lesions, with special reference to the FVPC.