Structural analysis of gene loci for rat U1 small nuclear RNA.
Structural analysis of gene loci for rat U1 small nuclear RNA.
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大鼠 U1 小核 RNA 基因位点的结构分析。
DOI：
10.1093/nar/11.6.1791
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发表时间：
1983
影响因子：
14.9
通讯作者：
Y. Ohshima
中科院分区：
文献类型：
--
作者：
N. Watanabe;Y. Itoh;T. Tani;K. Okano;N. Koga;N. Okada;Y. Ohshima
从大鼠基因文库中获得了4个能与U1小核RNA杂交的噬菌体克隆。两个克隆含有一个假定的假基因。第三个克隆包括两个与大鼠U1-RNA共线性的候选基因，相距3.6kb，方向相反。这两个基因被上游491 bp和下游178 bp的相同序列包围。上游序列不含TATA盒，但与人U1-RNA基因（1-3）有许多同源性。一个101 bp的“标识符（ID）序列”，据报道在大鼠脑中特异性表达（4），被插入紧接在一个基因下游的共享序列之后。在第四个克隆中，有两个假定的假基因，它们有一个或三个核苷酸的变化，相距3 kb，并在相同的方向。对大鼠总DNA的Southern印迹分析揭示了基因组中约50个U1-RNA基因/假基因。
Four phage clones which hybridize with U1 small nuclear RNA were obtained from a rat gene library. Two clones contain a presumed pseudogene. A third clone includes two gene candidates that are co-linear with the rat U1-RNA, 3.6kb apart and in the opposite orientation. The two genes are surrounded by identical sequences of 491bp upstream and 178bp downstream. The upstream sequences do not contain a TATA box, but share many block homologies with those for the human U1-RNA gene(1-3). A 101bp "identifier (ID) sequence", which was reported to be specifically expressed in rat brain (4), is inserted immediately after the shared sequence downstream of one of the genes. In the fourth clone, there are two putative pseudogenes, which have one or three nucleotide changes, 3kb apart and in the same orientation. Southern blot analysis of total rat DNA reveals about 50 U1-RNA genes/pseudogenes in the genome.