Expression of a cathelicidin antimicrobial peptide is augmented in cholesteatoma
Expression of a cathelicidin antimicrobial peptide is augmented in cholesteatoma
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DOI：
10.1097/00005537-200303000-00008
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发表时间：
2003-03-01
期刊：
影响因子：
2.6
通讯作者：
Hwang, SJ
中科院分区：
文献类型：
--
作者：
Jung, HH;Chae, SW;Hwang, SJ
目的/假设：抗菌肽是天然免疫的主动防御成分。它们在上皮表面作为预防感染的直接屏障效应的重要性得到了证实。放线菌素是一种多肽抗生素，正受到越来越多的关注。一些研究表明，过度表达长春花碱可以增强对细菌感染的保护，防止微生物的局部感染和全身入侵。这项研究的目的是调查长春瑞定是否上调。胆脂瘤上皮与正常皮肤比较。研究设计来自胆脂瘤组织和正常皮肤的前瞻性研究的20名患者参加了这项研究。标本被分成两部分。一部分用于后续的RNA研究，另一部分用于免疫组织化学染色。方法：研究方法。逆转录-聚合酶链式反应检测胆脂瘤组织和正常皮肤组织中肠毒素信使RNA的表达水平。推定浓度的中草药和β(2)-微球蛋白的mRNA被评估。分析各组的β(2-)微球蛋白与淫羊藿苷的比值。应用免疫组织化学方法检测胆脂瘤和正常皮肤上皮中胆脂素的表达。结果。胆脂瘤上皮中肠毒素基因的表达是正常耳道皮肤的5.5倍。在……里面。在胆脂瘤上皮中，放线菌素表达于各层，而在正常皮肤中仅在颗粒层和棘细胞层表达。结论：放线菌素在胆脂瘤上皮细胞中表达增强，本研究的数据与放线菌素可能是胆脂瘤表面上皮细胞第一道防线的关键成分的假设一致。
Objectives/Hypothesis: Antimicrobial peptides are active defense components of innate immunity. Their importance was confirmed at epithelial surfaces as immediate barrier effectors in preventing infection. Cathelicidins are peptide antibiotics that are receiving increasing attention. Several studies have shown that overexpression of cathelicidin results in augmented protection against bacterial infection and prevention of local infection and systemic invasion of microbes. The goal of the study was to investigate whether cathelicidin is upregulated. in cholesteatoma epithelium compared with normal skin. Study Design Twenty patients from a prospective study of cholesteatoma tissues and normal skins were enrolled in the study. The specimens were divided into two portions. One portion was used for subsequent RNA studies; the other was used for imimmohistochemical staining. Methods. Reverse transcriptase-polymerase chain reaction was used to assess the expression levels of cathelicidin messenger RNA (mRNA) both in cholesteatoma and in normal skin. Presumptive concentration of cathelicidin mRNA and beta(2)-microglobulin mRNA was evaluated. Ratio of beta(2-)microglobulin to cathelicidin was analyzed in each group. The expressions of cathelicidin in cholesteatoma and normal skin epithelium were investigated by an immunohistochemical technique. Results. Cathelicidin mRNA in cholesteatoma epithelium was increased 5.5-fold compared with normal skin of the ear canal. In. cholesteatoma epithelium, cathelicidin was located in all the layers, but in the normal skin it was expressed only in the granular and prickle cell layers. Conclusions: Cathelicidin is augmented in cholesteatoma epithelium, and the data in the present study are in agreement with the hypothesis that cathelicidin is likely to act as a key component in the first line of defense at the surface epithelium.