Self-adaptive antibacterial surface with bacterium-triggered antifouling-bactericidal switching properties
Self-adaptive antibacterial surface with bacterium-triggered antifouling-bactericidal switching properties
复制标题
具有细菌触发防污-杀菌切换特性的自适应抗菌表面
DOI：
10.1039/c9bm01666j
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发表时间：
2020
影响因子：
6.6
通讯作者：
Fu-Jian Xu
中科院分区：
文献类型：
--
作者：
Yidan Zhang;Xiang Zhang;Yu-Qing Zhao;Xin-Yang Zhang;Xiaokang Ding;Xuejia Ding;Bingran Yu;Shun Duan;Fu-Jian Xu
导管引起的感染是临床实践中的一个严重问题，会导致显着的发病率、死亡率和治疗费用。因此，开发抗菌表面的导管材料有很大的需求。在本研究中，我们开发了一种通过表面引发原子转移自由基聚合（SI-ATRP）在聚氨酯（PU）上构建具有细菌触发防污-杀菌切换特性的自适应抗菌表面的策略。以聚[2-(二甲基癸基铵)乙基甲基丙烯酸酯](PQDMAEMA)刷作为杀菌下层，聚乙二醇(PEG)作为防污上层，在PU表面(PU-PQ-PEG)上容易构建了一种具有分层结构的聚合物涂层。这两层与席夫碱结构结合在一起，可以被细菌的新陈代谢破坏。在正常和轻度感染条件下，PU-PQ-PEG对蛋白质和细菌表现出优异的防污和生物相容性。当发生严重感染且细菌定植在PU-PQ-PEG表面时，细菌会触发表面的自适应防污-杀菌切换。此外，模拟流体动力学条件的体外循环模型也证实了PU-PQ-PEG的自适应抗菌特性。 PU-PQ-PEG在静态和流体动力条件下均表现出自适应抗菌性能。动物实验结果也证明了其体内抗感染性能。目前的工作将为开发具有血液相容性的导管材料抗菌表面提供有前景的策略。
Catheter-induced infection is a severe problem in clinical practice, which induces significant morbidity, mortality and treatment costs. Therefore, there is a great requirement for developing antibacterial surfaces of catheter materials. In the present study, we develop a strategy for constructing self-adaptive antibacterial surfaces with bacterium-triggered antifouling-bactericidal switching properties on polyurethane (PU) via surface-initiated atom-transfer radical polymerization (SI-ATRP). Polymer coating with one hierarchical structure was readily constructed on the PU surface (PU-PQ-PEG), which was composed of poly[2-(dimethyl decyl ammonium)ethyl methacrylate] (PQDMAEMA) brushes as the bactericidal lower layer and polyethylene glycol (PEG) as the antifouling upper layer. The two layers were incorporated with Schiff base structures, which could be broken by the metabolism of bacteria. Under normal and mild infection conditions, PU-PQ-PEG showed excellent antifouling and biocompatible properties against proteins and bacteria. When serious infection occurred and bacteria colonized on the PU-PQ-PEG surface, the bacteria could trigger the self-adaptive antifouling-bactericidal switching of the surface. Furthermore, the self-adaptive antibacterial properties of PU-PQ-PEG were also confirmed by an in vitro circulating model to simulate hydrodynamic conditions. PU-PQ-PEG showed self-adaptive antibacterial performances both under static and hydrodynamic conditions. The results of animal experiments also demonstrated the in vivo anti-infection performance. The present work will provide a promising strategy for developing antibacterial surfaces of catheter materials with hemocompatibility.