Expression of a DEFICIENS-like gene correlates with the differentiation between sepal and petal in the orchid, Habenaria radiata (Orchidaceae)
Expression of a DEFICIENS-like gene correlates with the differentiation between sepal and petal in the orchid, Habenaria radiata (Orchidaceae)
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DOI：
10.1016/j.plantsci.2006.09.009
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发表时间：
2007-02-01
期刊：
影响因子：
5.2
通讯作者：
Kanno, Akira
中科院分区：
文献类型：
--
作者：
Kim, So-Young;Yun, Pil-Yong;Kanno, Akira
B 类 MADS-box 基因在控制双子叶植物花瓣发育中发挥着重要作用，并且已从许多植物中分离出直系同源物。兰科包括许多种，第一轮有花瓣状萼片，也有几个属有两轮花被、花瓣和绿色萼片。由于萼片和花瓣之间的形态分化在不同的兰科植物（如杓兰和瓤）中观察到，因此这一特征在兰科植物中将独立进化。 Habenaria radiata 是一种观赏植物，其花被上有绿色的萼片和白色的花瓣。在H. radiata品种中，一种花同源异型突变体的轮1发生了从萼片到花瓣状器官的转变。为了研究兰科植物萼片和花瓣形态分化的分子机制，分析H. radiata野生型和花瓣状萼片突变体中B类基因的表达是非常有趣的。在这项研究中，我们从该物种中分离和鉴定了三个 B 类基因：HrGLO1、HrGLO2 和 HrDEF。 Northern杂交和RT-PCR分析表明，HrGLO1和HrGLO2在萼片、花瓣和柱中表达，而HrDEF仅在花瓣和柱中检测到表达，而不在第一轮、萼片中检测到。在花瓣状萼片突变体中，所有三个B类基因均在花瓣状萼片、花瓣和柱中表达。这些结果表明，HrDEF 的独特表达在辐射花萼片和花瓣的分化中发挥作用。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
The B-class MADS-box genes play an important role in controlling petal development in dicots, and orthologs have been isolated from many plants. The Orchidaceae includes many species with petaloid sepals in whorl 1, while there are also several genera with two whorls of perianth, petals and greenish sepals. Since the morphological differentiation between sepals and petals is observed in different orchid groups such as Cypripedium and Habenaria, this characteristic would be evolved independently in Orchidaceae. Habenaria radiata, which is an ornamental plant, has green sepals and white petals in its perianth. Among the cultivars of H. radiata, one floral homeotic mutant has undergone an alteration from sepals to petaloid organs in whorl 1. In order to investigate the molecular mechanism of the morphological differentiation between sepals and petals in Orchidaceae, it is very interesting to analyze the B-class gene expression in wild type and petaloid-sepal mutant of H. radiata. In this study, we isolated and characterized three B-class genes, HrGLO1, HrGLO2 and HrDEF, from this species. Northern hybridization and RT-PCR analyses revealed that HrGLO1 and HrGLO2 were expressed in sepals, petals and columns, whereas HrDEF expression was detected only in the petals and columns, but not in the first whorl, sepals. In the petaloid-sepal mutant, all three B-class genes were expressed in petaloid-sepals, petals and columns. These results suggest that the distinctive expression of HrDEF plays a role in differentiation between sepal and petal in H. radiata. (c) 2006 Elsevier Ireland Ltd. All rights reserved.