Hydrophilization of bixin by lipase-catalyzed transesterification with sorbitol
Hydrophilization of bixin by lipase-catalyzed transesterification with sorbitol
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DOI：
10.1016/j.foodchem.2018.06.085
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发表时间：
2018-12-01
期刊：
影响因子：
8.8
通讯作者：
Dalsgaard, Trine Kastrup
中科院分区：
文献类型：
--
作者：
Jahangiri, Amita;Moller, Anders Hauer;Dalsgaard, Trine Kastrup
胭脂红是食品工业中使用最多的橙黄色食用色素之一。胭脂树红的多烯链使其具有高度疏水性，不太适合水基食品配方。以胭脂树红和山梨醇为原料，在脂肪酶催化下合成了一种具有潜在亲水性的胭脂树红衍生物。有趣的是，我们表明，胭脂树红的脂肪酶催化反应导致酯交换反应和形成的酯交换产物，山梨醇酯的降胭脂树红（SEN）。在有机溶剂2-甲基-2-丁醇中，以不同的水活度水平优化了各种固定化脂肪酶的反应效率。在所检查的脂肪酶中，固定化的南极假丝酵母脂肪酶B（Novozyme 435）在接近于零的水活度下提供最高的反应产率。使用四氢呋喃（THF）作为共溶剂以改善胭脂树红溶解度。在20%THF的条件下，SEN的总收率为50%。
Bixin is one of the most used yellow-orange food colorants in the food industry. The polyene chain of bixin makes it highly hydrophobic and less suitable for water-based food formulations. Lipase-catalyzed reactions of bixin with sorbitol were studied to synthesize a new derivative of bixin with potential hydrophilic properties. Interestingly, we show that the lipase-catalyzed reaction of bixin leads to a transesterification reaction and formation of a transesterified product, sorbitol ester of norbixin (SEN). The reaction efficiency was optimized with various immobilized lipases at different water activity levels in the organic solvent, 2-methyl-2-butanol. Among the examined lipases, immobilized Candida antarctica lipase B (Novozyme 435) provided the highest reaction yield at a water activity close to zero. Tetrahydrofuran (THF) was used as co-solvent to improve bixin solubility. The optimization of the reaction conditions with 20% THF lead to a total reaction yield of 50% of SEN.