A New Procedure for the Determination of Chromic Oxide with Potassium Phosphate Reagent
A New Procedure for the Determination of Chromic Oxide with Potassium Phosphate Reagent
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磷酸钾试剂测定氧化铬的新方法
DOI：
10.2141/jpsa.4.24
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发表时间：
1967
影响因子：
1.5
通讯作者：
K. Kameoka
中科院分区：
文献类型：
--
作者：
Minoru Yoshida;K. Kosaka;S. Horii;K. Kameoka
在鸡等动物的消化代谢研究中，常以氧化铬（Cr2 O3）为指标。在牛奶中矿物质的营养研究中，一位作者发现，惰性Cr2 O3与牛奶灰一起加热变得可溶于水。因此，本研究旨在寻找有效的化学物质，并建立一种新的分析方法来测定Cr2 O3与这些化学物质进行。经磷酸钾和氢氧化钾处理后的家禽饲料和排泄物中Cr_2O_3的吸收曲线与CrO_4的吸收曲线一致。在家禽饲料和排泄物中未发现影响三氧化二铬测定准确度的因素。试剂：将50 g K3 PO 4和25 g KOH用蒸馏水溶解成100 ml。称取含有约1.5毫克Cr2 O3的样品放入坩埚中。3.向样品中加入1 ml试剂溶液，混合并燃烧碳化。如有必要，在碳化前干燥样品-试剂混合物。4.将样品-试剂混合物在800°C的电烘箱中加热30分钟。冷却后，用蒸馏水溶解混合物，转移至容量瓶中，定容至250 ml。然后连夜离开了。过滤溶液，用370 m μ紫外线测定溶液的光密度。
Chromic oxide (Cr2O3) is used as an index in the research works on digestion and metabolism with chickens and other animals. During the nutritional studies on minerals in milk, one of the authors found that inert Cr2O3 heated with milk ash became watersoluble. Accordingly, studies to find out the effective chemicals in milk ash and to establish a new analytical procedure for the determination of Cr2O3 with these chemicals were carried out. It was found that the absorption curve of Cr2O3 in the poultry feed and excreta treated by the following procedure with potassium phosphate and potassium hydroxide was identical to that of CrO4". No factors in common poultry feed and excreta was found to affect the accuracy of Cr2O3 determination.New Procedure1. Reagent: 50g of K3PO4 and 25g of KOH are solved with distilled water into 100ml.2. Sample containing approximately 1.5mg of Cr2O3 is weighed into crucible.3. One ml of reagent solution is added to the sample, mixed and burned for carbonization. If necessary, dry the sample-reagent mixture before the carbonization.4. Sample-reagent mixture is heated in an electric oven at 800°C for 30 minutes.5. After cooling, the mixture is solved with distilled water, transferred into volumetric flask, filled up to 250ml. and left overnight.6. The solution is filtered off and optical density of the solution is determined with ultraviolet ray of 370mμ.